 
REBASE version 501                                              azymes.501
 
    =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
    REBASE, The Restriction Enzyme Database   http://rebase.neb.com
    Copyright (c)  Dr. Richard J. Roberts, 2014.   All rights reserved.
    =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
 
Rich Roberts                                                    Dec 30 2014
 
 AarI                                    CACCTGC(4/8)           2     F                                               
 AatII                                   GACGT^C                2     F,I,K,M,N,V                                     
                     AspJI               GACGTC                                                                       
                     Ppu1253I            GACGTC                                                                       
                     Ssp5230I            GACGT^C                                                                      
                     ZraI                GAC^GTC                      I,N,V                                           
 AbaCIII                                 CTATCAV                2                                                     
 AbsI                                    CC^TCGAGG              2     I                                               
 AccI                                    GT^(A/C)(G/T)AC        2     B,J,K,M,N,Q,R,S,X                               
                     DsaVI               GT(A/C)(G/T)AC                                                               
                     FblI                GT^(A/C)(G/T)AC              I,V                                             
                     OmiBII              GT(A/C)(G/T)AC                                                               
                     XmiI                GT^(A/C)(G/T)AC              F                                               
 AceIII                                  CAGCTC(7/11)           2                                                     
 AciI                                    CCGC(-3/-1)            2     N                                               
                     BspACI              CCGC(-3/-1)                  I                                               
                     SsiI                CCGC(-3/-1)                  F                                               
 AclI                                    AA^CGTT                2     I,N,V                                           
                     Psp1406I            AA^CGTT                      F,K                                             
                     UbaN9I              AACGTT                                                                       
 AcyI                                    GPu^CGPyC              2     J                                               
                     AhaII               GPu^CGPyC                                                                    
                     AosII               GPu^CGPyC                                                                    
                     AstWI               GPu^CGPyC                                                                    
                     AsuIII              GPu^CGPyC                                                                    
                     BbiII               GPu^CGPyC                                                                    
                     BsaHI               GPu^CGPyC                    N                                               
                     BssNI               GPu^CGPyC                    V                                               
                     BstACI              GPu^CGPyC                    I                                               
                     HgiI                GPu^CGPyC                                                                    
                     HgiDI               GPu^CGPyC                                                                    
                     HgiGI               GPu^CGPyC                                                                    
                     HgiHII              GPu^CGPyC                                                                    
                     Hin1I               GPu^CGPyC                    F,K                                             
                     Hin8I               GPuCGPyC                                                                     
                     Hsp92I              GPu^CGPyC                    R                                               
                     Msp17I              GPu^CGPyC                                                                    
                     NlaSII              GPuCGPyC                                                                     
                     PamII               GPu^CGPyC                                                                    
                     SspJII              GPuCGPyC                                                                     
                     SspM1II             GPuCGPyC                                                                     
                     SspM2II             GPuCGPyC                                                                     
                     Uba1381I            GPuCGPyC                                                                     
 AflII                                   C^TTAAG                2     J,K,N                                           
                     BfrI                C^TTAAG                      M                                               
                     BsaFI               CTTAAG                                                                       
                     BscLI               CTTAAG                                                                       
                     BspTI               C^TTAAG                      F                                               
                     Bst98I              C^TTAAG                                                                      
                     BstAFI              C^TTAAG                      I                                               
                     BstPZ740I           C^TTAAG                                                                      
                     Cfr92I              CTTAAG                                                                       
                     Esp4I               C^TTAAG                                                                      
                     MspCI               C^TTAAG                      C                                               
                     TrsTII              CTTAAG                                                                       
                     Uba1266I            CTTAAG                                                                       
                     Uba1299I            CTTAAG                                                                       
                     Uba1312I            CTTAAG                                                                       
                     Uba1313I            CTTAAG                                                                       
                     Uba1331I            CTTAAG                                                                       
                     Uba1374I            CTTAAG                                                                       
                     Uba1420I            CTTAAG                                                                       
                     Uba1426I            CTTAAG                                                                       
                     Uba1443I            CTTAAG                                                                       
                     VfiI                CTTAAG                                                                       
                     Vha464I             C^TTAAG                      V                                               
 AflIII                                  A^CPuPyGT              2     M,N                                             
                     Asp90I              ACPuPyGT                                                                     
 AgeI                                    A^CCGGT                2     J,N,R                                           
                     AsiAI               A^CCGGT                                                                      
                     AsiGI               A^CCGGT                      I,V                                             
                     BshTI               A^CCGGT                      F                                               
                     CsiAI               A^CCGGT                                                                      
                     CspAI               A^CCGGT                      C                                               
                     PinAI               A^CCGGT                      Q,X                                             
 AgsI                                    TT(C/G)^AA             2     I                                               
 AhaIII                                  TTT^AAA                2                                                     
                     DraI                TTT^AAA                      B,F,I,J,K,M,N,Q,R,S,U,V,X                       
                     PauAII              TTT^AAA                                                                      
                     Srl19I              TTTAAA                                                                       
                     Srl61DI             TTTAAA                                                                       
                     Srl76DI             TTTAAA                                                                       
                     SruI                TTT^AAA                                                                      
 AjuI                                    (7/12)GAANNNNNNNTTGG(11/6)2     F                                               
 AlfI                                    (10/12)GCANNNNNNTGC(12/10)2     F                                               
 AloI                                    (7/12)GAACNNNNNNTCC(12/7)2     F                                               
 AluI                                    AG^CT                  2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X                   
                     AluBI               AG^CT                        I                                               
                     BsaLI               AGCT                                                                         
                     MarI                AGCT                                                                         
                     Mho2111I            AGCT                                                                         
                     MltI                AG^CT                                                                        
                     OtuI                AGCT                                                                         
                     OtuNI               AGCT                                                                         
                     OxaI                AGCT                                                                         
                     Uba1433I            AGCT                                                                         
                     Uba1441I            AGCT                                                                         
 AlwFI                                   GAAAYNNNNNRTG          2                                                     
 AlwNI                                   CAGNNN^CTG             2     N                                               
                     CaiI                CAGNNN^CTG                   F                                               
                     PstNI               CAGNNN^CTG                   I                                               
 ApaI                                    GGGCC^C                2     B,F,I,J,K,M,N,Q,R,S,U,V,X                       
                     Bsp120I             G^GGCCC                      F                                               
                     EciEI               GGGCCC                                                                       
                     PpeI                GGGCC^C                                                                      
                     Psp30I              GGGCCC                                                                       
                     PspOMI              G^GGCCC                      I,N,V                                           
                     Uba1156I            GGGCCC                                                                       
                     Uba1157I            GGGCCC                                                                       
                     Uba1165I            GGGCCC                                                                       
                     Uba1202I            GGGCCC                                                                       
                     Uba1241I            GGGCCC                                                                       
                     Uba1368I            GGGCCC                                                                       
 ApaBI                                   GCANNNNN^TGC           2                                                     
                     BstAPI              GCANNNN^NTGC                 I,N                                             
 ApaLI                                   G^TGCAC                2     C,K,N                                           
                     AaqI                GTGCAC                                                                       
                     Alw44I              G^TGCAC                      F,J                                             
                     AmeI                GTGCAC                                                                       
                     Bsp146I             GTGCAC                                                                       
                     DaqI                GTGCAC                                                                       
                     Pfl23I              GTGCAC                                                                       
                     Pfr12I              GTGCAC                                                                       
                     PliI                GTGCAC                                                                       
                     ScoNI               GTGCAC                                                                       
                     SnoI                G^TGCAC                                                                      
                     Uba1203I            GTGCAC                                                                       
                     Uba1387I            GTGCAC                                                                       
                     VneI                G^TGCAC                      I,V                                             
 ApoI                                    Pu^AATTPy              2     N                                               
                     AcsI                Pu^AATTPy                    I,V                                             
                     CfaI                Pu^AATTPy                                                                    
                     FsiI                Pu^AATTPy                                                                    
                     XapI                Pu^AATTPy                    F                                               
 ApyPI                                   ATCGAC(20/18)          2                                                     
 AquII                                   GCCGNAC(20/18)         2                                                     
 AquIV                                   GRGGAAG(19/17)         2                                                     
 ArsI                                    (8/13)GACNNNNNNTTYG(11/6)2     I                                               
 AscI                                    GG^CGCGCC              2     N                                               
                     PalAI               GG^CGCGCC                    I                                               
                     SgsI                GG^CGCGCC                    F                                               
 AsuI                                    G^GNCC                 2                                                     
                     AhaB1I              GGNCC                                                                        
                     ApuI                GGNCC                                                                        
                     AspS9I              G^GNCC                       I,V                                             
                     AvcI                G^GNCC                                                                       
                     Bac36I              G^GNCC                                                                       
                     Bal228I             G^GNCC                                                                       
                     BavAII              G^GNCC                                                                       
                     BavBII              G^GNCC                                                                       
                     Bce22I              G^GNCC                                                                       
                     Bfi105I             GGNCC                                                                        
                     BmgT120I            G^GNCC                       K                                               
                     BpsI                GGNCC                                                                        
                     BsaSI               GGNCC                                                                        
                     Bse54I              GGNCC                                                                        
                     BshKI               G^GNCC                                                                       
                     BsiZI               G^GNCC                                                                       
                     Bsp1894I            G^GNCC                                                                       
                     BspBII              G^GNCC                                                                       
                     BspF4I              G^GNCC                                                                       
                     Bsu54I              G^GNCC                                                                       
                     Bsu5044I            GGNCC                                                                        
                     CcuI                G^GNCC                                                                       
                     CdiAI               GGNCC                                                                        
                     CdiCD6I             GGNCC                                                                        
                     Cfr4I               GGNCC                                                                        
                     Cfr8I               GGNCC                                                                        
                     Cfr13I              G^GNCC                       F                                               
                     Cfr23I              GGNCC                                                                        
                     Cfr33I              GGNCC                                                                        
                     Cfr45I              GGNCC                                                                        
                     Cfr46I              GGNCC                                                                        
                     Cfr47I              GGNCC                                                                        
                     Cfr52I              GGNCC                                                                        
                     Cfr54I              GGNCC                                                                        
                     CfrNI               GGNCC                                                                        
                     Eco39I              GGNCC                                                                        
                     Eco47II             GGNCC                                                                        
                     Eco196II            GGNCC                                                                        
                     Eco201I             GGNCC                                                                        
                     FmuI                GGNC^C                                                                       
                     GseI                GGNCC                                                                        
                     Hin5II              GGNCC                                                                        
                     HpyF66I             GGNCC                                                                        
                     HpyF71II            GGNCC                                                                        
                     HpyF73IV            GGNCC                                                                        
                     MaeK81II            G^GNCC                                                                       
                     MhoI                GGNCC                                                                        
                     MjaII               GGNCC                                                                        
                     Msp24I              GGNCC                                                                        
                     MthBI               GGNCC                                                                        
                     NlaDII              GGNCC                                                                        
                     NmuEII              GGNCC                                                                        
                     NmuSI               GGNCC                                                                        
                     NspIV               G^GNCC                                                                       
                     Nsp7121I            G^GNCC                                                                       
                     NspLII              GGNCC                                                                        
                     Pde12I              G^GNCC                                                                       
                     Pph1579I            GGNCC                                                                        
                     Pph1773I            GGNCC                                                                        
                     PseI                GGNCC                                                                        
                     PspI                GGNCC                                                                        
                     PspPI               G^GNCC                       C                                               
                     Sau2I               GGNCC                                                                        
                     Sau5I               GGNCC                                                                        
                     Sau13I              GGNCC                                                                        
                     Sau14I              GGNCC                                                                        
                     Sau17I              GGNCC                                                                        
                     Sau32I              GGNCC                                                                        
                     Sau33I              GGNCC                                                                        
                     Sau96I              G^GNCC                       J,N                                             
                     Sau557I             GGNCC                                                                        
                     SauBI               GGNCC                                                                        
                     SdyI                GGNCC                                                                        
                     SelAI               GGNCC                                                                        
                     TdeIII              GGNCC                                                                        
                     Uba1099I            GGNCC                                                                        
                     Uba1134I            GGNCC                                                                        
                     Uba1160I            GGNCC                                                                        
                     Uba1164I            GGNCC                                                                        
                     UnbI                ^GGNCC                                                                       
                     VchO66I             GGNCC                                                                        
                     VchO85I             GGNCC                                                                        
                     VchO90I             GGNCC                                                                        
                     VpaK15I             GGNCC                                                                        
                     VpaK25I             GGNCC                                                                        
                     VpaK9AI             GGNCC                                                                        
                     VpaK19AI            GGNCC                                                                        
                     VpaK19BI            GGNCC                                                                        
                     VpaKutAI            GGNCC                                                                        
                     VpaKutBI            GGNCC                                                                        
                     VpaKutJI            GGNCC                                                                        
 AsuII                                   TT^CGAA                2     C                                               
                     AcaI                TTCGAA                                                                       
                     AcpI                TT^CGAA                                                                      
                     Afl83I              TTCGAA                                                                       
                     Asp10HI             TT^CGAA                                                                      
                     AviI                TTCGAA                                                                       
                     BimI                TT^CGAA                                                                      
                     Bim19I              TT^CGAA                                                                      
                     Bpu14I              TT^CGAA                      I,V                                             
                     BpuHI               TTCGAA                                                                       
                     BsiCI               TT^CGAA                                                                      
                     Bsp82I              TTCGAA                                                                       
                     Bsp90I              TTCGAA                                                                       
                     Bsp101I             TTCGAA                                                                       
                     Bsp102I             TTCGAA                                                                       
                     Bsp104I             TTCGAA                                                                       
                     Bsp119I             TT^CGAA                      F                                               
                     Bsp148I             TTCGAA                                                                       
                     Bsp151I             TTCGAA                                                                       
                     Bsp241I             TTCGAA                                                                       
                     BspH22I             TTCGAA                                                                       
                     BspH103I            TTCGAA                                                                       
                     BspH106I            TTCGAA                                                                       
                     BspLAII             TT^CGAA                                                                      
                     BspT104I            TT^CGAA                      K                                               
                     BstBI               TT^CGAA                      N                                               
                     CbiI                TT^CGAA                                                                      
                     Csp45I              TT^CGAA                                                                      
                     Csp68KII            TT^CGAA                                                                      
                     FspII               TT^CGAA                                                                      
                     LspI                TT^CGAA                                                                      
                     MlaI                TT^CGAA                                                                      
                     Mva16I              TTCGAA                                                                       
                     NspV                TT^CGAA                      J                                               
                     Nsp29132I           TTCGAA                                                                       
                     NspBI               TTCGAA                                                                       
                     NspFI               TTCGAA                                                                       
                     NspJI               TTCGAA                                                                       
                     OspI                TTCGAA                                                                       
                     PlaII               TT^CGAA                                                                      
                     PpaAI               TT^CGAA                                                                      
                     Psc27I              TTCGAA                                                                       
                     Psc28I              TTCGAA                                                                       
                     Rma376I             TTCGAA                                                                       
                     Rma523I             TTCGAA                                                                       
                     SfuI                TT^CGAA                      M                                               
                     Sgr1839I            TTCGAA                                                                       
                     Ssp1I               TT^CGAA                                                                      
                     Ssp14I              TTCGAA                                                                       
                     Ssp34I              TTCGAA                                                                       
                     Ssp42I              TTCGAA                                                                       
                     Ssp43I              TTCGAA                                                                       
                     Ssp45I              TTCGAA                                                                       
                     Ssp47I              TTCGAA                                                                       
                     Ssp48I              TTCGAA                                                                       
                     Ssp152I             TTCGAA                                                                       
                     SspRFI              TT^CGAA                                                                      
                     SviI                TT^CGAA                                                                      
                     Uba1385I            TTCGAA                                                                       
                     Uba1452I            TTCGAA                                                                       
 AvaI                                    C^PyCGPuG              2     J,N,Q,U,X                                       
                     AcrI                CPyCGPuG                                                                     
                     Ama87I              C^PyCGPuG                    I,V                                             
                     AquI                C^PyCGPuG                                                                    
                     AspBI               CPyCGPuG                                                                     
                     AspDI               CPyCGPuG                                                                     
                     AvrI                CPyCGPuG                                                                     
                     BcoI                C^PyCGPuG                                                                    
                     BmeT110I            C^PyCGPuG                    B,K                                             
                     Bse15I              C^PyCGPuG                                                                    
                     BsiHKCI             C^PyCGPuG                    Q,X                                             
                     BsoBI               C^PyCGPuG                    N                                               
                     BspLU4I             C^PyCGPuG                                                                    
                     BstBAII             CPyCGPuG                                                                     
                     BstNSII             CPyCGPuG                                                                     
                     Bst7QI              CPyCGPuG                                                                     
                     BstSI               C^PyCGPuG                                                                    
                     BstZ4I              CPyCGPuG                                                                     
                     Eco88I              C^PyCGPuG                    F                                               
                     Eco27kI             C^PyCGPuG                                                                    
                     EspHK29I            CPyCGPuG                                                                     
                     Gst1588I            CPyCGPuG                                                                     
                     NliI                CPyCGPuG                                                                     
                     Nli3877I            CPyCGPu^G                                                                    
                     NmuAI               CPyCGPuG                                                                     
                     NspIII              C^PyCGPuG                                                                    
                     NspDI               CPyCGPuG                                                                     
                     NspEI               CPyCGPuG                                                                     
                     NspSAI              C^PyCGPuG                                                                    
                     OfoI                C^PyCGPuG                                                                    
                     PlaAI               C^PyCGPuG                                                                    
                     PunAI               C^PyCGPuG                                                                    
                     Uba1205II           CPyCGPuG                                                                     
                     Uba1436I            CPyCGPuG                                                                     
                     Uba1440I            CPyCGPuG                                                                     
                     UbaN6II             CPyCGPuG                                                                     
                     Umi5I               CPyCGPuG                                                                     
 AvaII                                   G^G(A/T)CC             2     J,N,R,X                                         
                     AflI                G^G(A/T)CC                                                                   
                     Asp697I             GG(A/T)CC                                                                    
                     Asp745I             G^G(A/T)CC                                                                   
                     AspBII              GG(A/T)CC                                                                    
                     AspDII              GG(A/T)CC                                                                    
                     BamNII              G^G(A/T)CC                                                                   
                     BceSII              GG(A/T)CC                                                                    
                     BcuAI               G^G(A/T)CC                                                                   
                     Bme18I              G^G(A/T)CC                   I,V                                             
                     Bme216I             G^G(A/T)CC                                                                   
                     BmpI                G^G(A/T)CC                                                                   
                     Bsp71I              GG(A/T)CC                                                                    
                     Bsp100I             GG(A/T)CC                                                                    
                     Bsp128I             GG(A/T)CC                                                                    
                     Bsp132I             GG(A/T)CC                                                                    
                     Bsp133I             GG(A/T)CC                                                                    
                     Bsp1260I            GG(A/T)CC                                                                    
                     BspF53I             GG(A/T)CC                                                                    
                     BspJ105I            GG(A/T)CC                                                                    
                     BsrAI               G^G(A/T)CC                                                                   
                     Bst4QI              GG(A/T)CC                                                                    
                     BthAI               G^G(A/T)CC                                                                   
                     BtiI                GG(A/T)CC                                                                    
                     CauI                G^G(A/T)CC                                                                   
                     CliI                GG(A/T)CC                                                                    
                     ClmII               GG(A/T)CC                                                                    
                     Csp68KI             G^G(A/T)CC                                                                   
                     DsaIV               G^G(A/T)CC                                                                   
                     EagMI               G^G(A/T)CC                                                                   
                     Eco47I              G^G(A/T)CC                   F                                               
                     ErpI                G^G(A/T)CC                                                                   
                     FdiI                G^G(A/T)CC                                                                   
                     FspMSI              G^G(A/T)CC                                                                   
                     FssI                G^G(A/T)CC                                                                   
                     GspAI               GG(A/T)CC                                                                    
                     HgiBI               G^G(A/T)CC                                                                   
                     HgiCII              G^G(A/T)CC                                                                   
                     HgiEI               G^G(A/T)CC                                                                   
                     HgiHIII             G^G(A/T)CC                                                                   
                     HgiJI               G^G(A/T)CC                                                                   
                     Hsp2I               GG(A/T)CC                                                                    
                     Kzo49I              G^G(A/T)CC                                                                   
                     Mae7806III          G^G(A/T)CC                                                                   
                     MfoI                GG(A/T)CC                                                                    
                     MliI                GG(A/T)CC                                                                    
                     MspAI               GG(A/T)CC                                                                    
                     MspNI               G^G(A/T)CC                                                                   
                     NliII               GG(A/T)CC                                                                    
                     Nli3877II           GG(A/T)CC                                                                    
                     NmuAII              GG(A/T)CC                                                                    
                     NspDII              GG(A/T)CC                                                                    
                     NspGI               GG(A/T)CC                                                                    
                     NspHII              GG(A/T)CC                                                                    
                     NspKI               GG(A/T)CC                                                                    
                     Pfl19I              GG(A/T)CC                                                                    
                     PolI                GG(A/T)CC                                                                    
                     Psp03I              GG(A/T)C^C                                                                   
                     SfnI                GG(A/T)CC                                                                    
                     Sgh1835I            GG(A/T)CC                                                                    
                     SinI                G^G(A/T)CC                   X                                               
                     SinAI               GG(A/T)CC                                                                    
                     SinBI               GG(A/T)CC                                                                    
                     SinCI               GG(A/T)CC                                                                    
                     SinDI               GG(A/T)CC                                                                    
                     SinEI               GG(A/T)CC                                                                    
                     SinFI               GG(A/T)CC                                                                    
                     SinGI               GG(A/T)CC                                                                    
                     SinHI               GG(A/T)CC                                                                    
                     SinJI               GG(A/T)CC                                                                    
                     SmuEI               G^G(A/T)CC                                                                   
                     SynI                GG(A/T)CC                                                                    
                     TruI                GG(A/T)CC                                                                    
                     Tru28I              GG(A/T)CC                                                                    
                     Tsp301I             GG(A/T)CC                                                                    
                     Uba48I              GG(A/T)CC                                                                    
                     Uba62I              GG(A/T)CC                                                                    
                     Uba1131I            GG(A/T)CC                                                                    
                     Uba1249I            GG(A/T)CC                                                                    
                     Uba1272I            GG(A/T)CC                                                                    
                     Uba1278I            GG(A/T)CC                                                                    
                     Uba1304I            GG(A/T)CC                                                                    
                     Uba1314I            GG(A/T)CC                                                                    
                     Uba1373I            GG(A/T)CC                                                                    
                     Uba1413I            GG(A/T)CC                                                                    
                     Uba1438I            GG(A/T)CC                                                                    
                     UbaN15I             GG(A/T)CC                                                                    
                     Uth554I             GG(A/T)CC                                                                    
                     VpaK11I             GG(A/T)CC                                                                    
                     VpaK65I             GG(A/T)CC                                                                    
                     VpaK7AI             GG(A/T)CC                                                                    
                     VpaK11AI            ^GG(A/T)CC                                                                   
                     VpaK13AI            GG(A/T)CC                                                                    
                     VpaK11BI            G^G(A/T)CC                   K                                               
                     VpaK11CI            GG(A/T)CC                                                                    
                     VpaK11DI            GG(A/T)CC                                                                    
 AvaIII                                  ATGCAT                 2                                                     
                     Asi372I             ATGCA^T                                                                      
                     BfrBI               ATGCA^T                                                                      
                     BfrCI               ATGCAT                                                                       
                     Csp68KIII           ATGCA^T                                                                      
                     EcoT22I             ATGCA^T                      B,K                                             
                     Mph1103I            ATGCA^T                      F                                               
                     NsiI                ATGCA^T                      J,M,N,Q,R,S,X                                   
                     PinBI               ATGCA^T                                                                      
                     Ppu10I              A^TGCAT                                                                      
                     PxyJKI              ATGCAT                                                                       
                     SepI                ATGCA^T                                                                      
                     SmuCI               ATGCAT                                                                       
                     SrlII               ATGCAT                                                                       
                     SspD5II             ATGCA^T                                                                      
                     Uba1353I            ATGCAT                                                                       
                     Uba1367I            ATGCAT                                                                       
                     Uba1384I            ATGCAT                                                                       
                     Zsp2I               ATGCA^T                      I,V                                             
 AvrII                                   C^CTAGG                2     N                                               
                     AspA2I              C^CTAGG                      I,V                                             
                     AvrBII              C^CTAGG                                                                      
                     BlnI                C^CTAGG                      K,M,S                                           
                     BspA2I              C^CTAGG                                                                      
                     XmaJI               C^CTAGG                      F                                               
 BaeI                                    (10/15)ACNNNNGTAYC(12/7)2     N                                               
 BalI                                    TGG^CCA                2     B,J,K,Q,R,X                                     
                     HauII               TGGCCANNNNNNNNNNN^                                                           
                     MlsI                TGG^CCA                      F                                               
                     Mlu31I              TGG^CCA                                                                      
                     MluNI               TGG^CCA                      M                                               
                     Mox20I              TGG^CCA                      I                                               
                     MscI                TGG^CCA                      N,O                                             
                     Msp16I              TGGCCA                                                                       
                     Msp20I              TGG^CCA                      V                                               
 BamHI                                   G^GATCC                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X,Y                 
                     AacLI               GGATCC                                                                       
                     AaeI                GGATCC                                                                       
                     AcaII               GGATCC                                                                       
                     AccEBI              G^GATCC                                                                      
                     AinII               GGATCC                                                                       
                     AliI                G^GATCC                                                                      
                     Ali12257I           GGATCC                                                                       
                     Ali12258I           GGATCC                                                                       
                     ApaCI               G^GATCC                                                                      
                     AsiI                G^GATCC                                                                      
                     AspTII              GGATCC                                                                       
                     Atu1II              GGATCC                                                                       
                     BamFI               GGATCC                                                                       
                     BamKI               GGATCC                                                                       
                     BamNI               GGATCC                                                                       
                     Bca1259I            GGATCC                                                                       
                     Bce751I             G^GATCC                                                                      
                     Bco10278I           GGATCC                                                                       
                     BnaI                G^GATCC                                                                      
                     BsaDI               GGATCC                                                                       
                     Bsp30I              GGATCC                                                                       
                     Bsp46I              GGATCC                                                                       
                     Bsp90II             GGATCC                                                                       
                     Bsp98I              G^GATCC                                                                      
                     Bsp130I             GGATCC                                                                       
                     Bsp131I             GGATCC                                                                       
                     Bsp144I             GGATCC                                                                       
                     Bsp4009I            G^GATCC                                                                      
                     BspAAIII            G^GATCC                                                                      
                     BstI                G^GATCC                                                                      
                     Bst1126I            GGATCC                                                                       
                     Bst2464I            GGATCC                                                                       
                     Bst2902I            GGATCC                                                                       
                     BstQI               GGATCC                                                                       
                     Bsu90I              GGATCC                                                                       
                     Bsu8565I            GGATCC                                                                       
                     Bsu8646I            GGATCC                                                                       
                     BsuB519I            GGATCC                                                                       
                     BsuB763I            GGATCC                                                                       
                     CelI                G^GATCC                                                                      
                     DdsI                GGATCC                                                                       
                     GdoI                GGATCC                                                                       
                     GinI                GGATCC                                                                       
                     GoxI                GGATCC                                                                       
                     GseIII              GGATCC                                                                       
                     GstI                G^GATCC                                                                      
                     MleI                GGATCC                                                                       
                     Mlu23I              G^GATCC                                                                      
                     NasBI               GGATCC                                                                       
                     Nsp29132II          G^GATCC                                                                      
                     NspSAIV             G^GATCC                                                                      
                     OkrAI               G^GATCC                                                                      
                     Pac1110I            GGATCC                                                                       
                     Pae177I             GGATCC                                                                       
                     PboCI               GGATCC                                                                       
                     Pfl8I               G^GATCC                                                                      
                     Psp56I              GGATCC                                                                       
                     RhsI                GGATCC                                                                       
                     Rlu4I               GGATCC                                                                       
                     RspLKII             G^GATCC                                                                      
                     SolI                G^GATCC                                                                      
                     SpvI                GGATCC                                                                       
                     SurI                G^GATCC                                                                      
                     Uba19I              GGATCC                                                                       
                     Uba31I              GGATCC                                                                       
                     Uba38I              GGATCC                                                                       
                     Uba51I              GGATCC                                                                       
                     Uba88I              GGATCC                                                                       
                     Uba1098I            GGATCC                                                                       
                     Uba1163I            GGATCC                                                                       
                     Uba1167I            GGATCC                                                                       
                     Uba1172I            GGATCC                                                                       
                     Uba1173I            GGATCC                                                                       
                     Uba1205I            GGATCC                                                                       
                     Uba1224I            GGATCC                                                                       
                     Uba1242I            GGATCC                                                                       
                     Uba1250I            GGATCC                                                                       
                     Uba1258I            GGATCC                                                                       
                     Uba1297I            GGATCC                                                                       
                     Uba1302I            GGATCC                                                                       
                     Uba1324I            GGATCC                                                                       
                     Uba1325I            GGATCC                                                                       
                     Uba1334I            GGATCC                                                                       
                     Uba1339I            GGATCC                                                                       
                     Uba1346I            GGATCC                                                                       
                     Uba1383I            GGATCC                                                                       
                     Uba1398I            GGATCC                                                                       
                     Uba1402I            GGATCC                                                                       
                     Uba1414I            GGATCC                                                                       
                     Uba4009I            G^GATCC                                                                      
                     UbaN3I              GGATCC                                                                       
                     UbaN14I             GGATCC                                                                       
 BanLI                                   RTCAGG                 2                                                     
 BarI                                    (7/12)GAAGNNNNNNTAC(12/7)2     I                                               
 BbvI                                    GCAGC(8/12)            2     N                                               
                     AlwXI               GCAGC(8/12)                                                                  
                     R1.BceSIV           (7/5)GCAGC(9/11)                                                             
                     R2.BceSIV           (7/5)GCAGC(9/11)                                                             
                     BchI                GCAGC                                                                        
                     BsaUI               GCAGC                                                                        
                     BseKI               GCAGC(8/12)                                                                  
                     BseXI               GCAGC(8/12)                  F                                               
                     Bsp423I             GCAGC(8/12)                                                                  
                     BsrVI               GCAGC                                                                        
                     Bst12I              GCAGC(8/12)                                                                  
                     Bst71I              GCAGC(8/12)                                                                  
                     BstV1I              GCAGC(8/12)                  I                                               
                     LfeI                GCAGC                                                                        
                     Lsp1109I            GCAGC(8/12)                  F                                               
 BbvII                                   GAAGAC(2/6)            2                                                     
                     Bbr7I               GAAGAC(7/11)                                                                 
                     BbsI                GAAGAC(2/6)                  N                                               
                     Bbv16II             GAAGAC(2/6)                                                                  
                     Bco102II            GAAGAC                                                                       
                     BpiI                GAAGAC(2/6)                  F                                               
                     BpuAI               GAAGAC(2/6)                                                                  
                     BsaVI               GAAGAC                                                                       
                     Bsc91I              GAAGAC(2/6)                                                                  
                     BscKI               GAAGAC                                                                       
                     BspBS31I            GAAGAC(2/6)                                                                  
                     BspIS4I             GAAGAC(2/6)                                                                  
                     BspTS514I           GAAGAC(2/6)                                                                  
                     BspVI               GAAGAC                                                                       
                     BstBS32I            GAAGAC(2/6)                                                                  
                     BstTS5I             GAAGAC(2/6)                                                                  
                     BstV2I              GAAGAC(2/6)                  I,V                                             
                     Rtr20I              GAAGAC                                                                       
 BbvCI                                   CCTCAGC(-5/-2)         2     N                                               
                     AbeI                CCTCAGC(-5/-2)                                                               
                     PmdI                CCTCAGC                                                                      
 BccI                                    CCATC(4/5)             2     N                                               
                     Hpy30XI             CCATC                                                                        
                     HpyC1I              CCATC(4/5)                                                                   
                     McaCI               CCATC                                                                        
                     MmyCVI              CCATC                                                                        
 Bce83I                                  CTTGAG(16/14)          2                                                     
                     BpuEI               CTTGAG(16/14)                N                                               
 BceJI                                   CACAG                  3                                                     
 BceSI                                   CGAAG(27/29)           3                                                     
 BcefI                                   ACGGC(12/13)           2                                                     
                     BceAI               ACGGC(12/14)                 N                                               
                     BceSIII             ACGGC(12/14)                                                                 
                     BctI                ACGGC                                                                        
 BcgI                                    (10/12)CGANNNNNNTGC(12/10)2     N                                               
 BciVI                                   GTATCC(6/5)            2     N                                               
                     BfuI                GTATCC(6/5)                  F                                               
                     BsuI                GTATCC(6/5)                  I                                               
                     LmoH7I              GTATCC                                                                       
 BclI                                    T^GATCA                2     C,F,J,M,N,O,R,S                                 
                     AbaI                T^GATCA                                                                      
                     AtuSI               TGATCA                                                                       
                     BceDI               TGATCA                                                                       
                     Bco102I             TGATCA                                                                       
                     BmeTI               TGATCA                                                                       
                     BsiQI               T^GATCA                                                                      
                     BspXII              T^GATCA                                                                      
                     Bst77I              TGATCA                                                                       
                     BstGI               TGATCA                                                                       
                     BstKI               TGATCA                                                                       
                     BstT7I              T^GATCA                                                                      
                     BstZ10II            TGATCA                                                                       
                     CpeI                TGATCA                                                                       
                     CthI                TGATCA                                                                       
                     FbaI                T^GATCA                      K                                               
                     Ksp22I              T^GATCA                      I,V                                             
                     ParI                T^GATCA                                                                      
                     PovI                TGATCA                                                                       
                     SseI                TGATCA                                                                       
                     SstIV               TGATCA                                                                       
                     Uba1282I            TGATCA                                                                       
                     Uba1283I            TGATCA                                                                       
                     Uba1431I            TGATCA                                                                       
                     Uba1447I            TGATCA                                                                       
                     Umi7I               TGATCA                                                                       
 BdaI                                    (10/12)TGANNNNNNTCA(12/10)2                                                     
 BetI                                    (A/T)^CCGG(A/T)        2                                                     
                     Bba179I             (A/T)CCGG(A/T)                                                               
                     Bca77I              (A/T)^CCGG(A/T)                                                              
                     BsaWI               (A/T)^CCGG(A/T)              N                                               
                     Bst1473I            (A/T)CCGG(A/T)                                                               
                     UbaN10I             (A/T)CCGG(A/T)                                                               
 BfaSII                                  GANGGAG                2                                                     
 BfiI                                    ACTGGG(5/4)            2                                                     
                     BmrI                ACTGGG(5/4)                  N                                               
                     BmuI                ACTGGG(5/4)                  I                                               
                     BsxI                ACTGGG                                                                       
 BglI                                    GCCNNNN^NGGC           2     C,F,I,J,K,N,O,Q,R,V,X                           
                     Bpu86I              GCCNNNNNGGC                                                                  
                     BseB631I            GCCNNNNNGGC                                                                  
                     BsoJI               GCCNNNNNGGC                                                                  
                     CagI                GCCNNNNNGGC                                                                  
                     Psp1009I            GCCNNNN^NGGC                                                                 
                     RspRSI              GCCNNNNNGGC                                                                  
                     Tsp219I             GCCNNNNNGGC                                                                  
                     Tsp8EI              GCCNNNN^NGGC                                                                 
                     VanI                GCCNNNNNGGC                                                                  
 BglII                                   A^GATCT                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X                   
                     BseB631II           AGATCT                                                                       
                     NcrI                A^GATCT                                                                      
                     NspMACI             A^GATCT                                                                      
                     Pae2kI              A^GATCT                                                                      
                     Pae18kI             A^GATCT                                                                      
 BinI                                    GGATC(4/5)             2                                                     
                     AclWI               GGATC(4/5)                   I                                               
                     AlwI                GGATC(4/5)                   N                                               
                     BpuFI               GGATC                                                                        
                     BspPI               GGATC(4/5)                   F                                               
                     BsrWI               GGATC                                                                        
                     BstH9I              GGATC(4/5)                                                                   
                     Bst31TI             GGATC(4/5)                                                                   
                     BthII               GGATC                                                                        
                     Bth617I             GGATC                                                                        
                     EacI                GGATC(4/5)                                                                   
                     Ral8I               GGATC                                                                        
 BmgI                                    GKGCCC                 2                                                     
 BplI                                    (8/13)GAGNNNNNCTC(13/8)2     F                                               
 Bpu10I                                  CCTNAGC(-5/-2)         2     F,I,N,V                                         
                     BpuDI               CCTNAGC(-5/-2)                                                               
 BsaAI                                   PyAC^GTPu              2     N                                               
                     BstBAI              PyAC^GTPu                    I,V                                             
                     MspYI               PyAC^GTPu                                                                    
                     Ppu6I               PyACGTPu                                                                     
                     Ppu11I              PyACGTPu                                                                     
                     Ppu21I              PyAC^GTPu                    F                                               
                     PsuAI               PyAC^GTPu                                                                    
 BsaBI                                   GATNN^NNATC            2     N                                               
                     Bco63I              GATNNNNATC                                                                   
                     Bco631I             GATNNNNATC                                                                   
                     Bse8I               GATNN^NNATC                  I,V                                             
                     Bse631I             GATNNNNATC                                                                   
                     BseJI               GATNN^NNATC                  F                                               
                     Bsh1365I            GATNN^NNATC                                                                  
                     BsiBI               GATNN^NNATC                                                                  
                     BsrBRI              GATNN^NNATC                                                                  
                     MamI                GATNN^NNATC                                                                  
                     UbaN6I              GATNNNNATC                                                                   
                     UbaN20I             GATNNNNATC                                                                   
 BsaXI                                   (9/12)ACNNNNNCTCC(10/7)2     N                                               
                     BsmCI               ACNNNNNCTCC                                                                  
                     BsmDI               ACNNNNNCTCC                                                                  
                     BsmXI               ACNNNNNCTCC                                                                  
 BsbI                                    CAACAC(21/19)          2                                                     
 BscGI                                   CCCGT                  2                                                     
                     BpuJI               CCCGT                                                                        
                     BstDZ247I           CCCGT                                                                        
 BseMII                                  CTCAG(10/8)            2     F                                               
                     BspCNI              CTCAG(9/7)                   N                                               
 BsePI                                   G^CGCGC                2     I,V                                             
                     BscEI               GCGCGC                                                                       
                     BsoPI               GCGCGC                                                                       
                     BsrHI               GCGCGC                                                                       
                     BssBI               GCGCGC                                                                       
                     BssHII              G^CGCGC                      J,K,M,N,Q,R,X                                   
                     BstBZ153I           G^CGCGC                                                                      
                     CfrJ5I              GCGCGC                                                                       
                     Eco143I             GCGCGC                                                                       
                     Eco152I             GCGCGC                                                                       
                     Esp7I               GCGCGC                                                                       
                     Esp8I               GCGCGC                                                                       
                     Kpn30I              GCGCGC                                                                       
                     McaTI               GCGC^GC                                                                      
                     PauI                G^CGCGC                      F                                               
                     PteI                G^CGCGC                      F                                               
                     TtmII               GCGCGC                                                                       
                     Uba69I              GCGCGC                                                                       
 BseRI                                   GAGGAG(10/8)           2     N                                               
                     AquIII              GAGGAG(20/18)                                                                
                     MchCM4I             GAGGAG                                                                       
                     SasI                GAGGAG                                                                       
 BseSI                                   G(G/T)GC(A/C)^C        2     F                                               
                     BaeGI               G(G/T)GC(A/C)^C              N                                               
                     Bme1580I            G(G/T)GC(A/C)^C                                                              
                     BmgAI               G(G/T)GC(A/C)C                                                               
                     BstSLI              G(G/T)GC(A/C)^C              I                                               
 BseYI                                   CCCAGC(-5/-1)          2     N                                               
                     GsaI                CCCAGC(-1/-5)                I                                               
                     PspFI               CCCAGC                                                                       
 BsgI                                    GTGCAG(16/14)          2     N                                               
 BsiI                                    CACGAG(-5/-1)          2                                                     
                     BauI                CACGAG(-5/-1)                F                                               
                     BssSI               CACGAG(-5/-1)                N                                               
                     Bst2BI              CACGAG(-5/-1)                I                                               
                     UbaN12I             CACGAG                                                                       
 BsiYI                                   CCNNNNN^NNGG           2                                                     
                     AfiI                CCNNNNN^NNGG                 V                                               
                     BflI                CCNNNNN^NNGG                                                                 
                     Bsc4I               CCNNNNN^NNGG                 I                                               
                     Bsc107I             CCNNNNN^NNGG                                                                 
                     Bse23I              CCNNNNNNNGG                                                                  
                     BseLI               CCNNNNN^NNGG                 F                                               
                     BslI                CCNNNNN^NNGG                 N                                               
                     BsmYI               CCNNNNNNNGG                                                                  
                     Bst22I              CCNNNNN^NNGG                                                                 
 BsmI                                    GAATGC(1/-1)           2     J,M,N,S                                         
                     Asp26HI             GAATGC(1/-1)                                                                 
                     Asp27HI             GAATGC(1/-1)                                                                 
                     Asp35HI             GAATGC(1/-1)                                                                 
                     Asp36HI             GAATGC(1/-1)                                                                 
                     Asp40HI             GAATGC(1/-1)                                                                 
                     Asp50HI             GAATGC(1/-1)                                                                 
                     BmaHI               GAATGC(1/-1)                                                                 
                     BsaMI               GAATGC(1/-1)                                                                 
                     BscCI               GAATGC(1/-1)                                                                 
                     Mva1269I            GAATGC(1/-1)                 F                                               
                     PctI                GAATGC(1/-1)                 I,V                                             
                     Uba1382I            GAATGC                                                                       
                     Uba1415I            GAATGC                                                                       
 BsmAI                                   GTCTC(1/5)             2     N                                               
                     Alw26I              GTCTC(1/5)                   F                                               
                     BcoDI               GTCTC(1/5)                   N                                               
                     BscQII              GTCTC                                                                        
                     BsoMAI              GTCTC(1/5)                                                                   
                     BstMAI              GTCTC(1/5)                   I,V                                             
                     Efa31KI             GTCTC                                                                        
 Bsp24I                                  (8/13)GACNNNNNNTGG(12/7)2                                                     
 Bsp1407I                                T^GTACA                2     F,K                                             
                     AauI                T^GTACA                                                                      
                     BsmGI               TGTACA                                                                       
                     BsmRI               T^GTACA                                                                      
                     BsrGI               T^GTACA                      N                                               
                     Bst170I             TGTACA                                                                       
                     BstAUI              T^GTACA                      I,V                                             
                     Ssp4800I            T^GTACA                                                                      
                     SspBI               T^GTACA                                                                      
 BspGI                                   CTGGAC                 2                                                     
 BspHI                                   T^CATGA                2     N                                               
                     CciI                T^CATGA                      I                                               
                     PagI                T^CATGA                      F                                               
                     RcaI                T^CATGA                                                                      
                     RhcI                TCATGA                                                                       
                     RspXI               T^CATGA                                                                      
 BspLU11I                                A^CATGT                2                                                     
                     PciI                A^CATGT                      I,N                                             
                     PscI                A^CATGT                      F                                               
 BspMI                                   ACCTGC(4/8)            2     N                                               
                     Acc36I              ACCTGC(4/8)                  I                                               
                     BfuAI               ACCTGC(4/8)                  N                                               
                     BveI                ACCTGC(4/8)                  F                                               
 BspMII                                  T^CCGGA                2                                                     
                     AccIII              T^CCGGA                      J,K,R                                           
                     Aor13HI             T^CCGGA                      K                                               
                     Bbf7411I            TCCGGA                                                                       
                     BbvAIII             T^CCGGA                                                                      
                     Bla7920I            TCCGGA                                                                       
                     BlfI                T^CCGGA                                                                      
                     BseAI               T^CCGGA                      C                                               
                     BsiGI               TCCGGA                                                                       
                     BsiMI               T^CCGGA                                                                      
                     BsiOI               TCCGGA                                                                       
                     Bsp13I              T^CCGGA                      I,V                                             
                     Bsp228I             TCCGGA                                                                       
                     Bsp233I             TCCGGA                                                                       
                     Bsp508I             TCCGGA                                                                       
                     BspEI               T^CCGGA                      N                                               
                     BspH226I            TCCGGA                                                                       
                     BstZ1I              TCCGGA                                                                       
                     BstZ3I              TCCGGA                                                                       
                     Bsu22I              TCCGGA                                                                       
                     Bsu23I              T^CCGGA                                                                      
                     CauB3I              T^CCGGA                                                                      
                     Cfr57I              TCCGGA                                                                       
                     EspHK26I            TCCGGA                                                                       
                     Kpn2I               T^CCGGA                      F                                               
                     MroI                T^CCGGA                      M,O                                             
                     PinBII              T^CCGGA                                                                      
                     PtaI                T^CCGGA                                                                      
                     Tsp507I             TCCGGA                                                                       
                     Tsp514I             TCCGGA                                                                       
                     Uba1136I            TCCGGA                                                                       
                     Uba1279I            TCCGGA                                                                       
                     Uba1375I            TCCGGA                                                                       
                     Uba1425I            TCCGGA                                                                       
 BspNCI                                  CCAGA                  2                                                     
 BsrI                                    ACTGG(1/-1)            2     N                                               
                     BscHI               ACTGG                                                                        
                     Bse1I               ACTGG(1/-1)                  I,V                                             
                     BseNI               ACTGG(1/-1)                  F                                               
                     BsoHI               ACTGG                                                                        
                     BsrSI               ACTGG(1/-1)                  R                                               
                     Bst11I              ACTGG(1/-1)                                                                  
                     Tsp1I               ACTGG(1/-1)                                                                  
 BsrBI                                   CCGCTC(-3/-3)          2     N                                               
                     AccBSI              CCGCTC(-3/-3)                I,V                                             
                     BstD102I            CCGCTC(-3/-3)                                                                
                     Bst31NI             CCGCTC(-3/-3)                                                                
                     MbiI                CCGCTC(-3/-3)                F                                               
 BsrDI                                   GCAATG(2/0)            2     N                                               
                     Bse3DI              GCAATG(2/0)                  I,V                                             
                     BseMI               GCAATG(2/0)                  F                                               
 BstEII                                  G^GTNACC               2     C,J,N,R,S                                       
                     AcrII               G^GTNACC                                                                     
                     AspAI               G^GTNACC                                                                     
                     Bse59I              GGTNACC                                                                      
                     Bse64I              G^GTNACC                                                                     
                     BseT9I              G^GTNACC                                                                     
                     BseT10I             G^GTNACC                                                                     
                     BsiKI               G^GTNACC                                                                     
                     Bsp1591I            GGTNACC                                                                      
                     Bst31I              GGTNACC                                                                      
                     BstDI               GGTNACC                                                                      
                     BstPI               G^GTNACC                     K                                               
                     BstT9I              G^GTNACC                                                                     
                     BstT10I             G^GTNACC                                                                     
                     Cfr7I               GGTNACC                                                                      
                     Cfr19I              GGTNACC                                                                      
                     EcaI                G^GTNACC                                                                     
                     Eci125I             G^GTNACC                                                                     
                     Eco91I              G^GTNACC                     F                                               
                     EcoO65I             G^GTNACC                     K                                               
                     EcoO128I            G^GTNACC                                                                     
                     KoxI                GGTNACC                                                                      
                     NspSAII             G^GTNACC                                                                     
                     PspEI               G^GTNACC                     I,V                                             
                     SciAI               GGTNACC                                                                      
                     Uba1291I            GGTNACC                                                                      
 BstXI                                   CCANNNNN^NTGG          2     F,I,J,K,M,N,Q,R,V,X,Y                           
                     BscJI               CCANNNNNNTGG                                                                 
                     BssGI               CCANNNNNNTGG                                                                 
                     BstHZ55I            CCANNNNN^NTGG                                                                
                     BstTI               CCANNNNNNTGG                                                                 
 BtgZI                                   GCGATG(10/14)          2     N                                               
 BtrI                                    CACGTC(-3/-3)          2     I                                               
                     AjiI                CACGTC(-3/-3)                F                                               
                     BmgBI               CACGTC(-3/-3)                N                                               
 Btr192II                                ACATC                  3                                                     
 BtsIMutI                                CAGTG(2/0)             2     N                                               
 BtsI                                    GCAGTG(2/0)            2     N                                               
 Cac8I                                   GCN^NGC                2     N                                               
                     BniI                GCNNGC                                                                       
                     BstC8I              GCN^NGC                      I                                               
 CauII                                   CC^(C/G)GG             2                                                     
                     AhaI                CC^(C/G)GG                                                                   
                     AseII               CC^(C/G)GG                                                                   
                     Asp1I               CC(C/G)GG                                                                    
                     AsuC2I              CC^(C/G)GG                   I                                               
                     BcnI                CC^(C/G)GG                   F,K                                             
                     BpuMI               CC^(C/G)GG                   V                                               
                     Bsp7I               CC(C/G)GG                                                                    
                     Bsp8I               CC(C/G)GG                                                                    
                     Bsp55I              CC(C/G)GG                                                                    
                     BspF105I            CC(C/G)GG                                                                    
                     BspJ67I             CC(C/G)GG                                                                    
                     EciDI               CC(C/G)GG                                                                    
                     Eco121I             CC(C/G)GG                                                                    
                     Eco179I             CC(C/G)GG                                                                    
                     Eco190I             CC(C/G)GG                                                                    
                     Eco1831I            ^CC(C/G)GG                                                                   
                     EcoHI               ^CC(C/G)GG                                                                   
                     HgiS21I             CC(C/G)GG                                                                    
                     HgiS22I             CC^(C/G)GG                                                                   
                     Hin3I               CC(C/G)GG                                                                    
                     Kpn49kII            ^CC(C/G)GG                                                                   
                     Mgl14481I           CC^(C/G)GG                                                                   
                     NciI                CC^(C/G)GG                   J,N,R                                           
                     Pae181I             CC(C/G)GG                                                                    
                     RshII               CC(C/G)GG                                                                    
                     SflHK10695I         CC(C/G)GG                                                                    
                     SflHK11086I         CC(C/G)GG                                                                    
                     SflHK11087I         CC(C/G)GG                                                                    
                     SflHK11572I         CC(C/G)GG                                                                    
                     SflHK115731I        CC(C/G)GG                                                                    
                     Ssp2I               CC(C/G)GG                                                                    
                     Tmu1I               CC(C/G)GG                                                                    
                     Uba41I              CC(C/G)GG                                                                    
                     Uba42I              CC(C/G)GG                                                                    
                     Uba1280I            CC(C/G)GG                                                                    
                     Uba1318I            CC(C/G)GG                                                                    
                     Uba1347I            CC(C/G)GG                                                                    
                     Uba1370I            CC(C/G)GG                                                                    
                     Uba1372I            CC(C/G)GG                                                                    
                     Uba1376I            CC(C/G)GG                                                                    
                     Uba1378I            CC(C/G)GG                                                                    
                     Uba1389I            CC(C/G)GG                                                                    
                     Uba1401I            CC(C/G)GG                                                                    
                     Uba1423I            CC(C/G)GG                                                                    
                     Uba1424I            CC(C/G)GG                                                                    
                     UbaN11I             CC(C/G)GG                                                                    
 CchII                                   GGARGA(11/9)           2                                                     
 CchIII                                  CCCAAG(20/18)          2                                                     
 CcrNAIII                                CGACCAG                2                                                     
 CdiI                                    CATCG(-1/-1)           2                                                     
 Cdi630V                                 CAAAAA                 2                                                     
 Cdi11397I                               GCGCAG                 2                                                     
 CdpI                                    GCGGAG(20/18)          2                                                     
                     NflHI               GCGGAG                                                                       
 CfrI                                    Py^GGCCPu              2                                                     
                     AcoI                Py^GGCCPu                    I                                               
                     Ava458I             PyGGCCPu                                                                     
                     Bfi89I              Py^GGCCPu                                                                    
                     Cfr14I              PyGGCCPu                                                                     
                     Cfr38I              PyGGCCPu                                                                     
                     Cfr39I              PyGGCCPu                                                                     
                     Cfr40I              PyGGCCPu                                                                     
                     Cfr55I              PyGGCCPu                                                                     
                     Cfr59I              PyGGCCPu                                                                     
                     EaeI                Py^GGCCPu                    K,N                                             
                     EciBI               PyGGCCPu                                                                     
                     Eco90I              PyGGCCPu                                                                     
                     Eco164I             PyGGCCPu                                                                     
                     EcoHAI              PyGGCCPu                                                                     
                     EcoHK31I            Py^GGCCPu                                                                    
                     EspHK16I            PyGGCCPu                                                                     
                     EspHK24I            PyGGCCPu                                                                     
                     KspHK15I            PyGGCCPu                                                                     
                     Uba36I              PyGGCCPu                                                                     
                     Uba1188I            PyGGCCPu                                                                     
                     Uba1327I            PyGGCCPu                                                                     
 Cfr10I                                  Pu^CCGGPy              2     F,K                                             
                     Bco118I             Pu^CCGGPy                                                                    
                     BscRI               PuCCGGPy                                                                     
                     Bse118I             Pu^CCGGPy                    I,V                                             
                     Bse634I             Pu^CCGGPy                                                                    
                     Bsp21I              PuCCGGPy                                                                     
                     BsrFI               Pu^CCGGPy                    N                                               
                     BssAI               Pu^CCGGPy                    C                                               
                     BstB7SI             Pu^CCGGPy                                                                    
                     NmeDI               (12/7)RCCGGY(7/12)                                                           
                     TseDI               PuCCGGPy                                                                     
 CfrAI                                   GCANNNNNNNNGTGG        1                                                     
 Cgl13032I                               GGCGCA                 2                                                     
 Cgl13032II                              ACGABGG                2                                                     
 CjeI                                    (8/14)CCANNNNNNGT(15/9)2                                                     
 CjeFII                                  CAAYNNNNNNACT          1                                                     
 CjeFIII                                 GCAAGG                 2                                                     
 CjeFV                                   GGRCA                  2                                                     
 CjeNII                                  GAGNNNNNGT             2                                                     
 CjeNIII                                 GKAAYG(19/17)          2                                                     
 CjeNIV                                  TAAYNNNNNTGC           1                                                     
                     CjeFIV              TAAYNNNNNTGC                                                                 
 CjePI                                   (7/13)CCANNNNNNNTC(14/8)2                                                     
 CjeP659IV                               CACNNNNNNNGAA          2                                                     
 CjuI                                    CAPyNNNNNPuTG          2                                                     
                     CcoP13III           CAPyNNNNNPuTG                                                                
                     HsoII               (19/14)CAYNNNNNRTG(-18/-25)                                                       
 CjuII                                   CAYNNNNNCTC            2                                                     
 ClaI                                    AT^CGAT                2     B,K,M,N,Q,R,S,U,X                               
                     AagI                AT^CGAT                                                                      
                     Apu16I              ATCGAT                                                                       
                     Asp14I              ATCGAT                                                                       
                     Asp37I              ATCGAT                                                                       
                     Asp86I              ATCGAT                                                                       
                     Asp123I             ATCGAT                                                                       
                     Asp130I             ATCGAT                                                                       
                     Asp707I             ATCGAT                                                                       
                     BanIII              AT^CGAT                                                                      
                     BavCI               AT^CGAT                                                                      
                     BazI                ATCGAT                                                                       
                     BbvAII              AT^CGAT                                                                      
                     Bci29I              AT^CGAT                                                                      
                     BciBI               AT^CGAT                                                                      
                     BcmI                AT^CGAT                                                                      
                     Bco79I              ATCGAT                                                                       
                     BdiI                AT^CGAT                                                                      
                     BfrAI               ATCGAT                                                                       
                     Bli41I              AT^CGAT                                                                      
                     Bli86I              AT^CGAT                                                                      
                     Bli576I             ATCGAT                                                                       
                     Bli585I             ATCGAT                                                                       
                     BliAI               AT^CGAT                                                                      
                     BliRI               AT^CGAT                                                                      
                     BpsII               ATCGAT                                                                       
                     Bsa29I              AT^CGAT                      I                                               
                     BscI                AT^CGAT                                                                      
                     BseCI               AT^CGAT                      C                                               
                     Bsh108AI            ATCGAT                                                                       
                     BshVI               AT^CGAT                      V                                               
                     BsiXI               AT^CGAT                                                                      
                     Bsp2I               ATCGAT                                                                       
                     Bsp4I               ATCGAT                                                                       
                     Bsp84I              ATCGAT                                                                       
                     Bsp106I             AT^CGAT                                                                      
                     Bsp125I             ATCGAT                                                                       
                     Bsp126I             ATCGAT                                                                       
                     Bsp127I             ATCGAT                                                                       
                     Bsp145I             ATCGAT                                                                       
                     BspDI               AT^CGAT                      N                                               
                     BspJII              AT^CGAT                                                                      
                     BspOVII             AT^CGAT                                                                      
                     BspXI               AT^CGAT                                                                      
                     BspZEI              AT^CGAT                                                                      
                     BsrCI               ATCGAT                                                                       
                     Bst28I              AT^CGAT                                                                      
                     BstLVI              ATCGAT                                                                       
                     BstNZ169I           AT^CGAT                                                                      
                     Bsu15I              AT^CGAT                      F                                               
                     BsuTUI              AT^CGAT                      X                                               
                     Bth1202I            ATCGAT                                                                       
                     Bth9415I            ATCGAT                                                                       
                     BtuI                ATCGAT                                                                       
                     Csp4I               ATCGAT                                                                       
                     LcaI                AT^CGAT                                                                      
                     LplI                AT^CGAT                                                                      
                     PgaI                AT^CGAT                                                                      
                     Rme21I              AT^CGAT                                                                      
                     SonI                ATCGAT                                                                       
                     SpmI                AT^CGAT                                                                      
                     Ssp27144I           AT^CGAT                                                                      
                     Uba22I              ATCGAT                                                                       
                     Uba24I              ATCGAT                                                                       
                     Uba30I              ATCGAT                                                                       
                     Uba34I              ATCGAT                                                                       
                     Uba43I              ATCGAT                                                                       
                     Uba1096I            ATCGAT                                                                       
                     Uba1100I            ATCGAT                                                                       
                     Uba1133I            ATCGAT                                                                       
                     Uba1137I            ATCGAT                                                                       
                     Uba1138I            ATCGAT                                                                       
                     Uba1144I            ATCGAT                                                                       
                     Uba1145I            ATCGAT                                                                       
                     Uba1161I            ATCGAT                                                                       
                     Uba1168I            ATCGAT                                                                       
                     Uba1195I            ATCGAT                                                                       
                     Uba1196I            ATCGAT                                                                       
                     Uba1197I            ATCGAT                                                                       
                     Uba1198I            ATCGAT                                                                       
                     Uba1199I            ATCGAT                                                                       
                     Uba1200I            ATCGAT                                                                       
                     Uba1233I            ATCGAT                                                                       
                     Uba1238I            ATCGAT                                                                       
                     Uba1246I            ATCGAT                                                                       
                     Uba1257I            ATCGAT                                                                       
                     Uba1275I            ATCGAT                                                                       
                     Uba1286I            ATCGAT                                                                       
                     Uba1295I            ATCGAT                                                                       
                     Uba1315I            ATCGAT                                                                       
                     Uba1342I            ATCGAT                                                                       
                     Uba1366II           ATCGAT                                                                       
                     Uba1379I            ATCGAT                                                                       
                     Uba1380I            ATCGAT                                                                       
                     Uba1394I            ATCGAT                                                                       
                     Uba1412I            ATCGAT                                                                       
                     Uba1416I            ATCGAT                                                                       
                     Uba1427I            ATCGAT                                                                       
                     Uba1430I            ATCGAT                                                                       
                     Uba1451I            ATCGAT                                                                       
                     Uba1453I            ATCGAT                                                                       
                     UbaN18I             ATCGAT                                                                       
                     UbaN22I             ATCGAT                                                                       
                     ZhoI                AT^CGAT                                                                      
 Csp2014I                                GGAGGC                 2                                                     
 CspCI                                   (11/13)CAANNNNNGTGG(12/10)2     N                                               
 CstMI                                   AAGGAG(20/18)          2                                                     
 CviJI                                   PuG^CPy                2     Q,X                                             
                     CviKI-1             PuG^CPy                      N                                               
                     CviKI               PuG^CPy                                                                      
                     CviLI               PuGCPy                                                                       
                     CviMI               PuGCPy                                                                       
                     CviNI               PuGCPy                                                                       
                     CviOI               PuGCPy                                                                       
 CviRI                                   TG^CA                  2                                                     
                     Hpy26I              TGCA                                                                         
                     HpyCH4V             TG^CA                        N                                               
                     HpyF36III           TGCA                                                                         
                     HpyF38II            TGCA                                                                         
                     HpyF44III           TG^CA                                                                        
                     HpyF45II            TGCA                                                                         
                     HpyF48III           TGCA                                                                         
                     HpyF49V             TGCA                                                                         
                     HpyF67II            TGCA                                                                         
                     HpyGII              TGCA                                                                         
                     HpyJP26I            TGCA                                                                         
 DdeI                                    C^TNAG                 2     K,M,N,O,Q,R,S,X                                 
                     Bpa36II             CTNAG                                                                        
                     BscPI               CTNAG                                                                        
                     Bst295I             CTNAG                                                                        
                     BstDEI              C^TNAG                       I,V                                             
                     BstJZ301I           C^TNAG                                                                       
                     HpyCH4II            CTNAG                                                                        
                     HpyF3I              C^TNAG                       F                                               
                     HpyF4II             CTNAG                                                                        
                     HpyF5I              CTNAG                                                                        
                     HpyF6III            CTNAG                                                                        
                     HpyF7I              CTNAG                                                                        
                     HpyF9II             CTNAG                                                                        
                     HpyF11I             CTNAG                                                                        
                     HpyF13II            CTNAG                                                                        
                     HpyF19I             CTNAG                                                                        
                     HpyF21I             CTNAG                                                                        
                     HpyF22II            CTNAG                                                                        
                     HpyF24II            CTNAG                                                                        
                     HpyF25I             CTNAG                                                                        
                     HpyF27I             CTNAG                                                                        
                     HpyF30II            CTNAG                                                                        
                     HpyF32I             CTNAG                                                                        
                     HpyF34I             CTNAG                                                                        
                     HpyF37I             CTNAG                                                                        
                     HpyF41II            CTNAG                                                                        
                     HpyF59I             CTNAG                                                                        
                     HpyF60II            CTNAG                                                                        
                     HpyF64V             CTNAG                                                                        
                     HpyF66II            CTNAG                                                                        
                     HpyF67I             CTNAG                                                                        
                     HpyF68II            CTNAG                                                                        
                     HpyF70I             CTNAG                                                                        
                     HpyF72II            CTNAG                                                                        
                     HpyHI               CTNAG                                                                        
                     HpyHPK5I            CTNAG                                                                        
 DraII                                   PuG^GNCCPy             2                                                     
                     EcoO109I            PuG^GNCCPy                   F,J,K,N                                         
                     Kaz48kI             PuGGNC^CPy                                                                   
                     PssI                PuGGNC^CPy                                                                   
                     Uba1326I            PuGGNCCPy                                                                    
                     VneAI               PuGGNCCPy                                                                    
 DraIII                                  CACNNN^GTG             2     I,M,N,V                                         
                     AdeI                CACNNN^GTG                   F                                               
                     BstIZ316I           CACNNN^GTG                                                                   
 DraRI                                   CAAGNAC(20/18)         2                                                     
 DrdI                                    GACNNNN^NNGTC          2     N                                               
                     AasI                GACNNNN^NNGTC                F                                               
                     DseDI               GACNNNN^NNGTC                I,V                                             
                     TrsSII              GACNNNNNNGTC                                                                 
 DrdII                                   GAACCA                 2                                                     
 DsaI                                    C^CPuPyGG              2                                                     
                     BstDSI              C^CPuPyGG                    I,V                                             
                     BtgI                C^CPuPyGG                    N                                               
                     TspBI               C^CPuPyGG                                                                    
 Eam1105I                                GACNNN^NNGTC           2     F,K                                             
                     AhdI                GACNNN^NNGTC                 N                                               
                     AspEI               GACNNN^NNGTC                                                                 
                     BmeRI               GACNNN^NNGTC                 V                                               
                     BspOVI              GACNNN^NNGTC                                                                 
                     BstZ2I              GACNNNNNGTC                                                                  
                     DriI                GACNNN^NNGTC                 I                                               
                     EclHKI              GACNNN^NNGTC                                                                 
                     NruGI               GACNNN^NNGTC                                                                 
                     Uba1190I            GACNNNNNGTC                                                                  
                     Uba1191I            GACNNNNNGTC                                                                  
 EciI                                    GGCGGA(11/9)           2     N                                               
                     BpuCI               GGCGGA                                                                       
 Eco31I                                  GGTCTC(1/5)            2     F                                               
                     Bli49I              GGTCTC                                                                       
                     Bli161I             GGTCTC                                                                       
                     Bli576II            GGTCTC                                                                       
                     Bli736I             GGTCTC(1/5)                                                                  
                     Bli5508I            GGTCTC                                                                       
                     BsaI                GGTCTC(1/5)                  N                                               
                     Bso31I              GGTCTC(1/5)                  I,V                                             
                     BspTNI              GGTCTC(1/5)                                                                  
                     Bsu537I             GGTCTC(1/5)                                                                  
                     Cfr56I              GGTCTC                                                                       
                     Eco42I              GGTCTC                                                                       
                     Eco51I              GGTCTC                                                                       
                     Eco95I              GGTCTC                                                                       
                     Eco97I              GGTCTC                                                                       
                     Eco101I             GGTCTC                                                                       
                     Eco120I             GGTCTC                                                                       
                     Eco127I             GGTCTC                                                                       
                     Eco129I             GGTCTC                                                                       
                     Eco155I             GGTCTC                                                                       
                     Eco156I             GGTCTC                                                                       
                     Eco157I             GGTCTC                                                                       
                     Eco162I             GGTCTC                                                                       
                     Eco185I             GGTCTC                                                                       
                     Eco191I             GGTCTC                                                                       
                     Eco203I             GGTCTC                                                                       
                     Eco204I             GGTCTC                                                                       
                     Eco205I             GGTCTC                                                                       
                     Eco217I             GGTCTC                                                                       
                     Eco225I             GGTCTC                                                                       
                     Eco233I             GGTCTC                                                                       
                     Eco239I             GGTCTC                                                                       
                     Eco240I             GGTCTC                                                                       
                     Eco241I             GGTCTC                                                                       
                     Eco246I             GGTCTC                                                                       
                     Eco247I             GGTCTC                                                                       
                     Eco263I             GGTCTC                                                                       
                     EcoA4I              GGTCTC(1/5)                                                                  
                     Eco71KI             GGTCTC                                                                       
                     EcoO44I             GGTCTC(1/5)                                                                  
                     PpaI                GGTCTC                                                                       
                     Rle69I              GGTCTC                                                                       
                     Sau12I              GGTCTC                                                                       
                     SlbI                GGTCTC                                                                       
                     Uba65I              GGTCTC                                                                       
                     Uba84I              GGTCTC                                                                       
                     Uba1316I            GGTCTC                                                                       
                     Uba1343I            GGTCTC                                                                       
                     VpaK57I             GGTCTC                                                                       
                     VpaK57AI            GGTCTC                                                                       
                     VpaKutHI            GGTCTC                                                                       
 Eco47III                                AGC^GCT                2     F,M,R                                           
                     AfeI                AGC^GCT                      I,N                                             
                     AitI                AGC^GCT                                                                      
                     Aor51HI             AGC^GCT                      K                                               
                     FunI                AGC^GCT                                                                      
 Eco57I                                  CTGAAG(16/14)          2     F                                               
                     AcuI                CTGAAG(16/14)                I,N                                             
                     Bsp6II              CTGAAG                                                                       
                     BspD6II             CTGAAG                                                                       
                     BspKT5I             CTGAAG(16/14)                                                                
                     Eco112I             CTGAAG                                                                       
                     Eco125I             CTGAAG                                                                       
                     FsfI                CTGAAG                                                                       
 Eco377I                                 GGANNNNNNNNATGC        1                                                     
                     Eco37I              GGANNNNNNNNATGC                                                              
                     Eco1323I            GGANNNNNNNNATGC                                                              
                     Eco1344II           GGANNNNNNNNATGC                                                              
                     Eco1386I            GGANNNNNNNNATGC                                                              
                     Eco1427I            GGANNNNNNNNATGC                                                              
                     Eco1430I            GGANNNNNNNNATGC                                                              
                     Eco1456I            GGANNNNNNNNATGC                                                              
                     Eco1476I            GGANNNNNNNNATGC                                                              
                     EcoAO83I            GGANNNNNNNNATGC                                                              
 Eco394I                                 GACNNNNNRTAAY          1                                                     
 Eco585I                                 GCCNNNNNNTGCG          1                                                     
 Eco646I                                 CCANNNNNNNCTTC         1                                                     
                     Eco1341I            CCANNNNNNNCTTC                                                               
                     Eco1383I            CCANNNNNNNCTTC                                                               
                     Eco1413I            CCANNNNNNNCTTC                                                               
                     Eco1422I            CCANNNNNNNCTTC                                                               
                     Eco1424I            CCANNNNNNNCTTC                                                               
                     Eco1432I            CCANNNNNNNCTTC                                                               
 Eco777I                                 GGANNNNNNTATC          1                                                     
                     Eco1348I            GGANNNNNNTATC                                                                
                     Eco1412I            GGANNNNNNTATC                                                                
 Eco826I                                 GCANNNNNNCTGA          1                                                     
                     Eco1455I            GCANNNNNNCTGA                                                                
 Eco851I                                 GTCANNNNNNTGAY         1                                                     
 Eco912I                                 CACNNNNNTGGC           1                                                     
 EcoAI                                   GAGNNNNNNNGTCA         1                                                     
                     Eco1446I            GAGNNNNNNNGTCA                                                               
 EcoBI                                   TGANNNNNNNNTGCT        1                                                     
                     Eco1158I            TGANNNNNNNNTGCT                                                              
                     Eco1265I            TGANNNNNNNNTGCT                                                              
                     Eco1441I            TGANNNNNNNNTGCT                                                              
                     Eco1443I            TGANNNNNNNNTGCT                                                              
                     Eco1444I            TGANNNNNNNNTGCT                                                              
                     Eco1447I            TGANNNNNNNNTGCT                                                              
 EcoDI                                   TTANNNNNNNGTCY         1                                                     
 EcoDR2                                  TCANNNNNNGTCG          1                                                     
 EcoDR3                                  TCANNNNNNNATCG         1                                                     
 EcoDXXI                                 TCANNNNNNNRTTC         1                                                     
 EcoEI                                   GAGNNNNNNNATGC         1                                                     
 EcoE1140I                               ACCYAC                 2                                                     
 EcoGVIII                                ACCACC                 3                                                     
 EcoKI                                   AACNNNNNNGTGC          1                                                     
                     Eco1342I            AACNNNNNNGTGC                                                                
                     Eco1344I            AACNNNNNNGTGC                                                                
                     Eco1394I            AACNNNNNNGTGC                                                                
 Eco57MI                                 CTGRAG(16/14)          2                                                     
 EcoNI                                   CCTNN^NNNAGG           2     N                                               
                     Bpu1268I            CCTNNNNNAGG                                                                  
                     BsoEI               CCTNNNNNAGG                                                                  
                     BstENI              CCTNN^NNNAGG                 I,V                                             
                     BstWI               CCTNNNNNAGG                                                                  
                     Uba1289I            CCTNNNNNAGG                                                                  
                     Uba1290I            CCTNNNNNAGG                                                                  
                     Uba1308I            CCTNNNNNAGG                                                                  
                     Uba1309I            CCTNNNNNAGG                                                                  
                     Uba1310I            CCTNNNNNAGG                                                                  
                     XagI                CCTNN^NNNAGG                 F                                               
 EcoPI                                   AGACC(25/27)           3                                                     
 EcoP15I                                 CAGCAG(25/27)          3     N                                               
 EcoRI                                   G^AATTC                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X,Y                 
                     Bci528I             GAATTC                                                                       
                     Eco82I              GAATTC                                                                       
                     Eco159I             GAATTC                                                                       
                     Eco228I             GAATTC                                                                       
                     Eco237I             GAATTC                                                                       
                     Eco252I             GAATTC                                                                       
                     FunII               G^AATTC                                                                      
                     HalI                G^AATTC                                                                      
                     Hal22I              GAATTC                                                                       
                     Kpn49kI             G^AATTC                                                                      
                     Ppu111I             G^AATTC                                                                      
                     RsrI                G^AATTC                                                                      
                     Srl32DII            G^AATTC                                                                      
                     Srl55DI             G^AATTC                                                                      
                     SsoI                G^AATTC                                                                      
                     Uba58I              GAATTC                                                                       
                     Van91II             GAATTC                                                                       
                     VchN100I            GAATTC                                                                       
                     VchO2I              GAATTC                                                                       
 EcoRII                                  ^CC(A/T)GG             2     F,J                                             
                     Acc38I              CC(A/T)GG                                                                    
                     AcuII               CC(A/T)GG                                                                    
                     AeuI                CC^(A/T)GG                                                                   
                     AglI                CC^(A/T)GG                                                                   
                     AjnI                ^CC(A/T)GG                   I                                               
                     AorI                CC^(A/T)GG                                                                   
                     ApaORI              CC^(A/T)GG                                                                   
                     ApyI                CC^(A/T)GG                                                                   
                     Asp2HI              CC(A/T)GG                                                                    
                     AtuII               CC(A/T)GG                                                                    
                     Atu1I               CC(A/T)GG                                                                    
                     AtuBI               CC(A/T)GG                                                                    
                     BciBII              CC^(A/T)GG                                                                   
                     BciT130I            CC^(A/T)GG                   K                                               
                     BinSI               CC(A/T)GG                                                                    
                     Bme2095I            CC(A/T)GG                                                                    
                     BptI                CC^(A/T)GG                                                                   
                     BsaNI               CC(A/T)GG                                                                    
                     Bse16I              CC^(A/T)GG                                                                   
                     Bse17I              CC^(A/T)GG                                                                   
                     Bse24I              CC^(A/T)GG                                                                   
                     BseBI               CC^(A/T)GG                   C                                               
                     BshGI               CC^(A/T)GG                                                                   
                     BsiLI               CC^(A/T)GG                                                                   
                     BsiUI               CC(A/T)GG                                                                    
                     BsiVI               CC(A/T)GG                                                                    
                     BsoGI               CC(A/T)GG                                                                    
                     Bsp44I              CC(A/T)GG                                                                    
                     Bsp56I              CC(A/T)GG                                                                    
                     Bsp103I             CC(A/T)GG                                                                    
                     Bsp317I             CC(A/T)GG                                                                    
                     BspH43I             CC(A/T)GG                                                                    
                     BspNI               CC^(A/T)GG                                                                   
                     BspSI               CC(A/T)GG                                                                    
                     Bst1I               CC^(A/T)GG                                                                   
                     Bst2I               CC^(A/T)GG                                                                   
                     Bst38I              CC^(A/T)GG                                                                   
                     Bst100I             CC^(A/T)GG                                                                   
                     BstGII              CC(A/T)GG                                                                    
                     BstM6I              CC^(A/T)GG                                                                   
                     BstNI               CC^(A/T)GG                   N                                               
                     BstOI               CC^(A/T)GG                   R                                               
                     Bst7QII             CC(A/T)GG                                                                    
                     Bst2UI              CC^(A/T)GG                   I,V                                             
                     BthDI               CC^(A/T)GG                                                                   
                     BthEI               CC^(A/T)GG                                                                   
                     CbrI                CC^(A/T)GG                                                                   
                     Cdi27I              CC(A/T)GG                                                                    
                     Cfr5I               CC(A/T)GG                                                                    
                     Cfr11I              CC(A/T)GG                                                                    
                     Cfr20I              CC(A/T)GG                                                                    
                     Cfr22I              CC(A/T)GG                                                                    
                     Cfr24I              CC(A/T)GG                                                                    
                     Cfr25I              CC(A/T)GG                                                                    
                     Cfr27I              CC(A/T)GG                                                                    
                     Cfr28I              CC(A/T)GG                                                                    
                     Cfr29I              CC(A/T)GG                                                                    
                     Cfr30I              CC(A/T)GG                                                                    
                     Cfr31I              CC(A/T)GG                                                                    
                     Cfr35I              CC(A/T)GG                                                                    
                     Cfr58I              CC(A/T)GG                                                                    
                     CfrS37I             CC(A/T)GG                                                                    
                     CthII               CC^(A/T)GG                                                                   
                     EagKI               CC(A/T)GG                                                                    
                     EcaII               CC(A/T)GG                                                                    
                     EclII               CC(A/T)GG                                                                    
                     Ecl66I              CC(A/T)GG                                                                    
                     Ecl136I             CC(A/T)GG                                                                    
                     Ecl137II            CC(A/T)GG                                                                    
                     EclS39I             CC(A/T)GG                                                                    
                     Ecl37kII            CC(A/T)GG                                                                    
                     Ecl54kI             CC(A/T)GG                                                                    
                     Ecl57kI             CC(A/T)GG                                                                    
                     Ecl1zII             CC(A/T)GG                                                                    
                     Eco38I              CC(A/T)GG                                                                    
                     Eco40I              CC(A/T)GG                                                                    
                     Eco41I              CC(A/T)GG                                                                    
                     Eco60I              CC(A/T)GG                                                                    
                     Eco61I              CC(A/T)GG                                                                    
                     Eco67I              CC(A/T)GG                                                                    
                     Eco70I              CC(A/T)GG                                                                    
                     Eco71I              CC(A/T)GG                                                                    
                     Eco128I             CC(A/T)GG                                                                    
                     Eco170I             CC(A/T)GG                                                                    
                     Eco193I             CC(A/T)GG                                                                    
                     Eco206I             CC(A/T)GG                                                                    
                     Eco207I             CC(A/T)GG                                                                    
                     Eco254I             CC(A/T)GG                                                                    
                     Eco256I             CC(A/T)GG                                                                    
                     Efa1KI              CC(A/T)GG                                                                    
                     Ese6II              CC(A/T)GG                                                                    
                     Esp2I               CC(A/T)GG                                                                    
                     Esp24I              CC(A/T)GG                                                                    
                     EspHK7I             CC(A/T)GG                                                                    
                     EspHK22I            CC(A/T)GG                                                                    
                     EspHK30I            CC(A/T)GG                                                                    
                     Fsp1604I            CC^(A/T)GG                                                                   
                     HhdI                CC(A/T)GG                                                                    
                     Kox165I             CC(A/T)GG                                                                    
                     Kpn10I              CC(A/T)GG                                                                    
                     Kpn13I              CC(A/T)GG                                                                    
                     Kpn14I              CC(A/T)GG                                                                    
                     Kpn16I              CC(A/T)GG                                                                    
                     KspHK12I            CC(A/T)GG                                                                    
                     KspHK14I            CC(A/T)GG                                                                    
                     Lla497I             CC(A/T)GG                                                                    
                     Mlu2300I            CC(A/T)GG                                                                    
                     MphI                CC(A/T)GG                                                                    
                     MpsI                CC(A/T)GG                                                                    
                     MvaI                CC^(A/T)GG                   F,M,S                                           
                     Pca17AI             CC(A/T)GG                                                                    
                     Psp6I               ^CC(A/T)GG                   I                                               
                     Psp39I              CC(A/T)GG                                                                    
                     PspGI               ^CC(A/T)GG                   N                                               
                     Sau16I              CC(A/T)GG                                                                    
                     Scg2I               CC(A/T)GG                                                                    
                     SelPI               CC(A/T)GG                                                                    
                     SflHK1794I          CC(A/T)GG                                                                    
                     SflHK2374I          CC(A/T)GG                                                                    
                     SflHK2731I          CC(A/T)GG                                                                    
                     SflHK6873I          CC(A/T)GG                                                                    
                     SflHK7234I          CC(A/T)GG                                                                    
                     SflHK7462I          CC(A/T)GG                                                                    
                     SflHK8401I          CC(A/T)GG                                                                    
                     SflHK10790I         CC(A/T)GG                                                                    
                     Sfl2aI              CC(A/T)GG                                                                    
                     Sfl2bI              CC(A/T)GG                                                                    
                     Sgr20I              CC(A/T)GG                                                                    
                     SleI                ^CC(A/T)GG                                                                   
                     SniI                CC^(A/T)GG                                                                   
                     SslI                CC^(A/T)GG                                                                   
                     SspAI               ^CC(A/T)GG                                                                   
                     Sth117I             CC^(A/T)GG                                                                   
                     Sth302I             CC^(A/T)GG                                                                   
                     Sth455I             CC(A/T)GG                                                                    
                     TaqXI               CC^(A/T)GG                                                                   
                     TspAI               CC(A/T)GG                                                                    
                     Uba11I              CC(A/T)GG                                                                    
                     Uba13I              CC(A/T)GG                                                                    
                     Uba20I              CC(A/T)GG                                                                    
                     Uba81I              CC(A/T)GG                                                                    
                     Uba82I              CC(A/T)GG                                                                    
                     Uba1114I            CC(A/T)GG                                                                    
                     Uba1118I            CC(A/T)GG                                                                    
                     Uba1120I            CC(A/T)GG                                                                    
                     Uba1121I            CC(A/T)GG                                                                    
                     Uba1125I            CC(A/T)GG                                                                    
                     Uba1171I            CC(A/T)GG                                                                    
                     Uba1181I            CC(A/T)GG                                                                    
                     Uba1185I            CC(A/T)GG                                                                    
                     Uba1189I            CC(A/T)GG                                                                    
                     Uba1193I            CC(A/T)GG                                                                    
                     Uba1218I            CC(A/T)GG                                                                    
                     Uba1243I            CC(A/T)GG                                                                    
                     Uba1410I            CC(A/T)GG                                                                    
                     Uba1428I            CC(A/T)GG                                                                    
                     UbaN11II            CC(A/T)GG                                                                    
                     UbaN16I             CC(A/T)GG                                                                    
                     ZanI                CC^(A/T)GG                                                                   
 EcoRV                                   GAT^ATC                2     B,C,I,J,K,M,N,O,Q,R,S,U,V,X                     
                     Bsc217I             GATATC                                                                       
                     BshLI               GATATC                                                                       
                     BsoAI               GATATC                                                                       
                     Bsp16I              GATATC                                                                       
                     BstRI               GATATC                                                                       
                     CeqI                GAT^ATC                                                                      
                     Eco17I              GATATC                                                                       
                     Eco32I              GAT^ATC                      F                                               
                     Eco178I             GATATC                                                                       
                     HjaI                GAT^ATC                                                                      
                     HpyCI               GAT^ATC                                                                      
                     NanI                GATATC                                                                       
                     NflAI               GATATC                                                                       
                     NsiCI               GAT^ATC                                                                      
                     Pac1110II           GATATC                                                                       
                     Pfl16I              GATATC                                                                       
                     PxyARI              GATATC                                                                       
                     Rma495II            GATATC                                                                       
                     Rma496II            GATATC                                                                       
                     Rma497II            GATATC                                                                       
                     SepMI               GAT^ATC                                                                      
                     SmiMII              GATATC                                                                       
                     Tsp273I             GATATC                                                                       
                     Uba1400I            GATATC                                                                       
                     UbaN4I              GATATC                                                                       
                     UbaN21I             GATATC                                                                       
 EcoR124I                                GAANNNNNNRTCG          1                                                     
 EcoR124II                               GAANNNNNNNRTCG         1                                                     
 EcoRD2                                  GAANNNNNNRTTC          1                                                     
 EcoRD3                                  GAANNNNNNNRTTC         1                                                     
 EcoprrI                                 CCANNNNNNNRTGC         1                                                     
 EsaSSI                                  GACCAC                 2                                                     
 EspI                                    GC^TNAGC               2                                                     
                     BlpI                GC^TNAGC                     N                                               
                     Bpu1102I            GC^TNAGC                     F,K                                             
                     BpuGCI              GCTNAGC                                                                      
                     Bsp1720I            GC^TNAGC                     I,V                                             
                     CelII               GC^TNAGC                                                                     
                     Uba1221I            GCTNAGC                                                                      
                     Uba1222I            GCTNAGC                                                                      
                     Uba1284I            GCTNAGC                                                                      
                     Uba1320I            GCTNAGC                                                                      
 Esp3I                                   CGTCTC(1/5)            2     F                                               
                     BsmBI               CGTCTC(1/5)                  N                                               
                     BstGZ53I            CGTCTC(1/5)                                                                  
                     Esp16I              CGTCTC                                                                       
                     Esp23I              CGTCTC                                                                       
 FaiI                                    PyA^TPu                2     I                                               
 FalI                                    (8/13)AAGNNNNNCTT(13/8)2     I                                               
 FauI                                    CCCGC(4/6)             2     I,N                                             
                     Bme585I             CCCGC(4/6)                                                                   
                     BstFZ438I           CCCGC(4/6)                                                                   
                     SmuI                CCCGC(4/6)                                                                   
 FinI                                    GGGAC                  2                                                     
                     BpuNI               GGGAC                                                                        
                     BpuSI               GGGAC(10/14)                                                                 
                     BscWI               GGGAC                                                                        
                     BslFI               GGGAC(10/14)                 I                                               
                     BsmFI               GGGAC(10/14)                 N                                               
                     BspLU11III          GGGAC(10/14)                                                                 
                     BstOZ616I           GGGAC(10/14)                                                                 
                     FaqI                GGGAC(10/14)                 F                                               
 FnuDII                                  CG^CG                  2                                                     
                     AccII               CG^CG                        J,K                                             
                     Bce31293I           CGCG                                                                         
                     BceBI               CG^CG                                                                        
                     BceRI               CGCG                                                                         
                     BepI                CG^CG                                                                        
                     Bpu95I              CG^CG                                                                        
                     Bsh1236I            CG^CG                        F                                               
                     Bsp50I              CG^CG                                                                        
                     Bsp70I              CGCG                                                                         
                     Bsp123I             CG^CG                                                                        
                     BspFNI              CG^CG                        I                                               
                     BspJ76I             CGCG                                                                         
                     BstFNI              CG^CG                        I,V                                             
                     BstUI               CG^CG                        N                                               
                     Bsu1192II           CGCG                                                                         
                     Bsu1193I            CGCG                                                                         
                     Bsu1532I            CG^CG                                                                        
                     Bsu6633I            CGCG                                                                         
                     BsuEII              CGCG                                                                         
                     BtkI                CG^CG                                                                        
                     Cpa1150I            CGCG                                                                         
                     Cpa6013I            CG^CG                                                                        
                     CpaAI               CGCG                                                                         
                     Csp68KVI            CG^CG                                                                        
                     EsaBS9I             CGCG                                                                         
                     FalII               CG^CG                                                                        
                     FauBII              CG^CG                                                                        
                     FspMI               CGCG                                                                         
                     Hin1056I            CGCG                                                                         
                     HpyF14I             CGCG                                                                         
                     HpyF15I             CGCG                                                                         
                     HpyF26I             CGCG                                                                         
                     HpyF52II            CGCG                                                                         
                     HpyF64IV            CGCG                                                                         
                     MvaAI               CGCG                                                                         
                     MvnI                CG^CG                        M                                               
                     PflAI               CGCG                                                                         
                     SceAI               CGCG                                                                         
                     SelI                ^CGCG                                                                        
                     ThaI                CG^CG                                                                        
                     TmaI                CGCG                                                                         
                     TneDI               CG^CG                                                                        
                     Uba1321I            CGCG                                                                         
                     Uba1404I            CGCG                                                                         
                     Uba1405I            CGCG                                                                         
                     Uba1446I            CGCG                                                                         
 Fnu4HI                                  GC^NGC                 2     N                                               
                     Bli1904II           GCNGC                                                                        
                     Bpu1811I            GCNGC                                                                        
                     BsoFI               GC^NGC                                                                       
                     Bsp6I               GC^NGC                                                                       
                     BssFI               GCNGC                                                                        
                     BssXI               GCNGC                                                                        
                     BthCI               GCNG^C                                                                       
                     Cac824I             GCNGC                                                                        
                     CcoP215I            GCNGC                                                                        
                     CcoP216I            GCNGC                                                                        
                     FbrI                GC^NGC                                                                       
                     Fsp4HI              GC^NGC                       I                                               
                     ItaI                GC^NGC                                                                       
                     LlaDII              GCNGC                                                                        
                     SatI                GC^NGC                       F                                               
                     Uur960I             GC^NGC                                                                       
 FokI                                    GGATG(9/13)            2     I,J,K,M,N,V,X,Y                                 
                     BseGI               GGATG(2/0)                   F                                               
                     BstF5I              GGATG(2/0)                   I,V                                             
                     BtsCI               GGATG(2/0)                   N                                               
                     HinGUII             GGATG                                                                        
                     Hpy178VI            GGATG                                                                        
                     HpyF6I              GGATG                                                                        
                     HpyF67III           GGATG                                                                        
                     PbuJKI              GGATG                                                                        
                     StsI                GGATG(10/14)                                                                 
 FseI                                    GGCCGG^CC              2     N                                               
                     RigI                GGCCGG^CC                    I                                               
 FspAI                                   PuTGC^GCAPy            2     F                                               
 GauT27I                                 CGCGCAGG               2                                                     
 Gba708II                                ATGCAC                 2                                                     
 GdiII                                   CGGCCR(-5/-1)          2                                                     
 GmeII                                   TCCAGG                 3                                                     
 GsuI                                    CTGGAG(16/14)          2     F                                               
                     Bco35I              CTGGAG                                                                       
                     BpmI                CTGGAG(16/14)                I,N                                             
                     Bsp22I              CTGGAG                                                                       
                     Bsp28I              CTGGAG                                                                       
                     BspJ74I             CTGGAG                                                                       
                     Bth1795I            CTGGAG                                                                       
                     Uba1437I            CTGGAG                                                                       
                     Uba1444I            CTGGAG                                                                       
 HaeI                                    (A/T)GG^CC(A/T)        2                                                     
 HaeII                                   PuGCGC^Py              2     J,K,N,R                                         
                     AccB2I              PuGCGC^Py                                                                    
                     BfoI                PuGCGC^Py                    F                                               
                     Bme142I             PuGC^GCPy                                                                    
                     BsmHI               PuGCGCPy                                                                     
                     Bsp143II            PuGCGC^Py                                                                    
                     Bst16I              PuGCGCPy                                                                     
                     Bst1473II           PuGCGCPy                                                                     
                     BstH2I              PuGCGC^Py                    I,V                                             
                     Btu34II             PuGCGCPy                                                                     
                     HinHI               PuGCGCPy                                                                     
                     LpnI                PuGC^GCPy                                                                    
                     NgoAI               PuGCGCPy                                                                     
                     NgoBI               PuGCGCPy                                                                     
                     NgoCI               PuGCGCPy                                                                     
                     NgoGI               PuGCGCPy                                                                     
                     NgoJI               PuGCGCPy                                                                     
                     NgoMI               PuGCGCPy                                                                     
                     NgoWI               PuGCGCPy                                                                     
 HaeIII                                  GG^CC                  2     B,I,J,K,M,N,O,Q,R,S,U,X                         
                     AcaIV               GGCC                                                                         
                     Afl83II             GGCC                                                                         
                     Asp742I             GGCC                                                                         
                     AspTIII             GGCC                                                                         
                     AvrBI               GGCC                                                                         
                     Bal475I             GGCC                                                                         
                     Bal3006I            GGCC                                                                         
                     BanAI               GG^CC                                                                        
                     Bce71I              GGCC                                                                         
                     Bco33I              GGCC                                                                         
                     BecAII              GG^CC                                                                        
                     Bfi458I             GGCC                                                                         
                     BhaII               GGCC                                                                         
                     Bim19II             GG^CC                                                                        
                     BliI                GGCC                                                                         
                     BluII               GGCC                                                                         
                     Bme46I              GGCC                                                                         
                     Bme74I              GGCC                                                                         
                     Bme361I             GG^CC                                                                        
                     BmeU1594I           GGCC                                                                         
                     Bpa36I              GGCC                                                                         
                     BsaRI               GGCC                                                                         
                     BscQI               GGCC                                                                         
                     BseI                GGCC                                                                         
                     Bse9I               GGCC                                                                         
                     Bse126I             GGCC                                                                         
                     BseQI               GG^CC                                                                        
                     BshI                GG^CC                                                                        
                     BshAI               GGCC                                                                         
                     BshBI               GGCC                                                                         
                     BshCI               GGCC                                                                         
                     BshDI               GGCC                                                                         
                     BshEI               GGCC                                                                         
                     BshFI               GG^CC                        C                                               
                     BsiAI               GGCC                                                                         
                     BsiDI               GGCC                                                                         
                     BsiHI               GGCC                                                                         
                     BsnI                GG^CC                        V                                               
                     Bsp23I              GGCC                                                                         
                     Bsp44II             GGCC                                                                         
                     Bsp137I             GGCC                                                                         
                     Bsp211I             GG^CC                                                                        
                     Bsp226I             GGCC                                                                         
                     Bsp881I             GGCC                                                                         
                     Bsp1261I            GGCC                                                                         
                     Bsp1593I            GGCC                                                                         
                     Bsp2013I            GGCC                                                                         
                     Bsp2362I            GGCC                                                                         
                     Bsp2500I            GGCC                                                                         
                     BspANI              GG^CC                                                                        
                     BspBDG2I            GGCC                                                                         
                     BspBRI              GG^CC                                                                        
                     BspBSE18I           GGCC                                                                         
                     BspBake1I           GGCC                                                                         
                     BspCHE15I           GGCC                                                                         
                     BspGHA1I            GGCC                                                                         
                     BspH106II           GGCC                                                                         
                     BspKI               GG^CC                                                                        
                     BspLRI              GGCC                                                                         
                     BspRI               GG^CC                                                                        
                     BssCI               GGCC                                                                         
                     BstCI               GGCC                                                                         
                     BstJI               GGCC                                                                         
                     Bsu1076I            GGCC                                                                         
                     Bsu1114I            GGCC                                                                         
                     BsuRI               GG^CC                        F,I                                             
                     BteI                GG^CC                                                                        
                     CbeI                GG^CC                                                                        
                     ClmI                GGCC                                                                         
                     CltI                GG^CC                                                                        
                     Csp2I               GGCC                                                                         
                     DsaII               GG^CC                                                                        
                     EsaBC4I             GG^CC                                                                        
                     EsaWC1I             GGCC                                                                         
                     FinSI               GGCC                                                                         
                     FnuDI               GG^CC                                                                        
                     HhgI                GGCC                                                                         
                     Hpy178VII           GGCC                                                                         
                     HpyF10V             GGCC                                                                         
                     HpyF26II            GGCC                                                                         
                     HpyF29I             GGCC                                                                         
                     HpyF33II            GGCC                                                                         
                     HpyF42I             GGCC                                                                         
                     HpyF46V             GGCC                                                                         
                     HpyF49IV            GGCC                                                                         
                     HpyF53I             GGCC                                                                         
                     HpyF57I             GGCC                                                                         
                     HpyF63I             GGCC                                                                         
                     HpyF69II            GGCC                                                                         
                     HpyF72I             GGCC                                                                         
                     HpyF73III           GGCC                                                                         
                     MchAII              GG^CC                                                                        
                     MfoAI               GG^CC                                                                        
                     MniI                GGCC                                                                         
                     MnnII               GGCC                                                                         
                     MthTI               GGCC                                                                         
                     NgoAII              GGCC                                                                         
                     NgoCII              GGCC                                                                         
                     NgoNII              GGCC                                                                         
                     NgoPII              GG^CC                                                                        
                     NgoSII              GGCC                                                                         
                     NgoTII              GGCC                                                                         
                     NlaI                GGCC                                                                         
                     NspLKI              GG^CC                                                                        
                     OchI                GGCC                                                                         
                     PaiI                GGCC                                                                         
                     PalI                GG^CC                                                                        
                     Pde133I             GG^CC                                                                        
                     PflKI               GG^CC                                                                        
                     PhoI                GG^CC                                                                        
                     PlaI                GG^CC                                                                        
                     Ple214I             GGCC                                                                         
                     PpuI                GGCC                                                                         
                     Pru2I               GG^CC                                                                        
                     Psb9879I            GGCC                                                                         
                     Psp29I              GGCC                                                                         
                     SagI                GGCC                                                                         
                     SbvI                GG^CC                                                                        
                     SfaI                GG^CC                                                                        
                     SplIII              GGCC                                                                         
                     SuaI                GG^CC                                                                        
                     SulI                GGCC                                                                         
                     Tsp132I             GGCC                                                                         
                     Tsp266I             GGCC                                                                         
                     Tsp273II            GGCC                                                                         
                     Tsp281I             GGCC                                                                         
                     Tsp560I             GGCC                                                                         
                     TspZNI              GGCC                                                                         
                     TteAI               GGCC                                                                         
                     TtnI                GGCC                                                                         
                     Tvu2HI              GG^CC                                                                        
                     Uba9I               GGCC                                                                         
                     Uba54I              GGCC                                                                         
                     Uba61I              GGCC                                                                         
                     Uba1097I            GGCC                                                                         
                     Uba1140I            GGCC                                                                         
                     Uba1146I            GGCC                                                                         
                     Uba1147I            GGCC                                                                         
                     Uba1150I            GGCC                                                                         
                     Uba1152I            GGCC                                                                         
                     Uba1153I            GGCC                                                                         
                     Uba1155I            GGCC                                                                         
                     Uba1169I            GGCC                                                                         
                     Uba1174I            GGCC                                                                         
                     Uba1175I            GGCC                                                                         
                     Uba1176I            GGCC                                                                         
                     Uba1178I            GGCC                                                                         
                     Uba1179I            GGCC                                                                         
                     Uba1207I            GGCC                                                                         
                     Uba1208I            GGCC                                                                         
                     Uba1209I            GGCC                                                                         
                     Uba1210I            GGCC                                                                         
                     Uba1214I            GGCC                                                                         
                     Uba1223I            GGCC                                                                         
                     Uba1228I            GGCC                                                                         
                     Uba1230I            GGCC                                                                         
                     Uba1231I            GGCC                                                                         
                     Uba1235I            GGCC                                                                         
                     Uba1288I            GGCC                                                                         
                     Uba1292I            GGCC                                                                         
                     Uba1293I            GGCC                                                                         
                     Uba1319I            GGCC                                                                         
                     Uba1322I            GGCC                                                                         
                     Uba1336I            GGCC                                                                         
                     Uba1377I            GGCC                                                                         
                     Uba1388I            GGCC                                                                         
                     Uba1392I            GGCC                                                                         
                     Uba1395I            GGCC                                                                         
                     Uba1408I            GGCC                                                                         
                     Uba1418I            GGCC                                                                         
                     Uba1422I            GGCC                                                                         
                     Uba1429I            GGCC                                                                         
                     Uba1449I            GGCC                                                                         
                     Uba1450I            GGCC                                                                         
                     UbaN2I              GGCC                                                                         
                     UbaN8I              GGCC                                                                         
                     Uth549I             GGCC                                                                         
                     Uth555I             GGCC                                                                         
                     Uth557I             GGCC                                                                         
                     Van91III            GGCC                                                                         
                     VhaI                GGCC                                                                         
                     Vha1168I            GGCC                                                                         
                     VniI                GGCC                                                                         
 HaeIV                                   (7/13)GAYNNNNNRTC(14/9)2                                                     
 HgaI                                    GACGC(5/10)            2     I,N                                             
                     CseI                GACGC(5/10)                  F                                               
                     NmeBI               GACGC                                                                        
 HgiAI                                   G(A/T)GC(A/T)^C        2                                                     
                     Alw21I              G(A/T)GC(A/T)^C              F                                               
                     AspHI               G(A/T)GC(A/T)^C                                                              
                     Bbv12I              G(A/T)GC(A/T)^C              I,V                                             
                     Bfu1570I            G(A/T)GC(A/T)C                                                               
                     BsaGI               G(A/T)GC(A/T)C                                                               
                     Bsh45I              G(A/T)GC(A/T)^C                                                              
                     BsiHKAI             G(A/T)GC(A/T)^C              N                                               
                     Bsm6I               G(A/T)GC(A/T)^C                                                              
                     BsmPI               G(A/T)GC(A/T)C                                                               
                     Hpy8III             G(A/T)GC(A/T)C                                                               
                     HpyF7II             G(A/T)GC(A/T)C                                                               
                     HpyF46II            G(A/T)GC(A/T)C                                                               
                     MspV281I            G(A/T)GC(A/T)^C                                                              
                     Pph3215I            G(A/T)GC(A/T)C                                                               
 HgiCI                                   G^GPyPuCC              2                                                     
                     AccB1I              G^GPyPuCC                    I,V                                             
                     BanI                G^GPyPuCC                    N,R                                             
                     BbvBI               G^GPyPuCC                                                                    
                     Bme05I              GGPyPuCC                                                                     
                     BshNI               G^GPyPuCC                    F                                               
                     BspT107I            G^GPyPuCC                    K                                               
                     Eco50I              GGPyPuCC                                                                     
                     Eco64I              G^GPyPuCC                                                                    
                     Eco168I             GGPyPuCC                                                                     
                     Eco169I             GGPyPuCC                                                                     
                     Eco171I             GGPyPuCC                                                                     
                     Eco173I             GGPyPuCC                                                                     
                     Eco195I             GGPyPuCC                                                                     
                     Esp1I               GGPyPuCC                                                                     
                     Esp6I               GGPyPuCC                                                                     
                     Esp9I               GGPyPuCC                                                                     
                     Esp10I              GGPyPuCC                                                                     
                     Esp11I              GGPyPuCC                                                                     
                     Esp12I              GGPyPuCC                                                                     
                     Esp13I              GGPyPuCC                                                                     
                     Esp14I              GGPyPuCC                                                                     
                     Esp15I              GGPyPuCC                                                                     
                     Esp21I              GGPyPuCC                                                                     
                     Esp22I              GGPyPuCC                                                                     
                     Esp25I              GGPyPuCC                                                                     
                     HgiHI               G^GPyPuCC                                                                    
                     MspB4I              G^GPyPuCC                                                                    
                     PfaAI               G^GPyPuCC                                                                    
                     Pph14I              GGPyPuCC                                                                     
                     SspM1III            GGPyPuCC                                                                     
                     Uba1127I            GGPyPuCC                                                                     
                     Vsp2246I            GGPyPuCC                                                                     
 HgiEII                                  ACCNNNNNNGGT           2                                                     
 HgiJII                                  GPuGCPy^C              2                                                     
                     BanII               GPuGCPy^C                    K,N,X                                           
                     BpuI                GPuGCPy^C                                                                    
                     BscMI               GPuGCPyC                                                                     
                     Bsp117I             GPuGCPyC                                                                     
                     Bsp519I             GPuGCPy^C                                                                    
                     BstZ7I              GPuGCPyC                                                                     
                     Bsu1854I            GPuGCPy^C                                                                    
                     BvuI                GPuGCPy^C                                                                    
                     Cfr48I              GPuGCPyC                                                                     
                     Eco24I              GPuGCPy^C                    F                                               
                     Eco25I              GPuGCPyC                                                                     
                     Eco26I              GPuGCPyC                                                                     
                     Eco35I              GPuGCPyC                                                                     
                     Eco68I              GPuGCPyC                                                                     
                     Eco113I             GPuGCPyC                                                                     
                     Eco180I             GPuGCPyC                                                                     
                     Eco211I             GPuGCPyC                                                                     
                     Eco215I             GPuGCPyC                                                                     
                     Eco216I             GPuGCPyC                                                                     
                     Eco232I             GPuGCPyC                                                                     
                     Eco249I             GPuGCPyC                                                                     
                     Eco262I             GPuGCPyC                                                                     
                     Eco75KI             GPuGCPy^C                                                                    
                     EcoT38I             GPuGCPy^C                    J                                               
                     EcoT88I             GPuGCPyC                                                                     
                     EcoT93I             GPuGCPyC                                                                     
                     EcoT95I             GPuGCPyC                                                                     
                     Ese4I               GPuGCPyC                                                                     
                     FriOI               GPuGCPy^C                    I,V                                             
                     KoxII               GPuGCPy^C                                                                    
                     OmiAI               GPuGCPyC                                                                     
                     OmiBI               GPuGCPyC                                                                     
                     PaeHI               GPuGCPy^C                                                                    
                     Ppu20I              GPuGCPyC                                                                     
                     Psp31I              GPuGCPyC                                                                     
                     SacNI               GPuGCPy^C                                                                    
                     Uba39I              GPuGCPyC                                                                     
                     Uba57I              GPuGCPyC                                                                     
                     Uba1124I            GPuGCPyC                                                                     
                     Uba1142I            GPuGCPyC                                                                     
                     Uba1159I            GPuGCPyC                                                                     
                     Uba1206I            GPuGCPyC                                                                     
                     Uba1263I            GPuGCPyC                                                                     
                     Uba1264I            GPuGCPyC                                                                     
                     Uba1307I            GPuGCPyC                                                                     
                     Uba1329I            GPuGCPyC                                                                     
                     Uba1330I            GPuGCPyC                                                                     
                     Uba1357I            GPuGCPyC                                                                     
                     Uba1363I            GPuGCPyC                                                                     
                     Uba1409I            GPuGCPyC                                                                     
                     Uba1421I            GPuGCPyC                                                                     
 HhaI                                    GCG^C                  2     B,F,J,K,N,Q,R,X                                 
                     AspLEI              GCG^C                        I,V                                             
                     BbtI                G^CGC                                                                        
                     BcaI                GCGC                                                                         
                     BspLAI              GCG^C                                                                        
                     BstHHI              GCG^C                        I,V                                             
                     CcoP95I             GCGC                                                                         
                     CfoI                GCG^C                        M,R,S                                           
                     Csp1470I            GCGC                                                                         
                     FnuDIII             GCG^C                                                                        
                     Hin6I               G^CGC                        F                                               
                     Hin7I               GCGC                                                                         
                     HinGUI              GCGC                                                                         
                     HinP1I              G^CGC                        N                                               
                     HinS1I              GCGC                                                                         
                     HinS2I              GCGC                                                                         
                     Hpy99III            GCGC                                                                         
                     HpyF10I             GCGC                                                                         
                     HsoI                G^CGC                                                                        
                     HspAI               G^CGC                        I,V                                             
                     Mho2965I            GCGC                                                                         
                     MnnIV               GCGC                                                                         
                     NgoEII              GCGC                                                                         
                     SciNI               G^CGC                                                                        
 Hin4I                                   (8/13)GAYNNNNNVTC(13/8)2                                                     
 Hin4II                                  CCTTC(6/5)             2                                                     
                     HpyAV               CCTTC(6/5)                   N                                               
 HindII                                  GTPy^PuAC              2     I,M,V                                           
                     ChuEII              GTPyPuAC                                                                     
                     Hin1160II           GTPyPuAC                                                                     
                     Hin1161II           GTPyPuAC                                                                     
                     HinJCI              GTPy^PuAC                                                                    
                     HincII              GTPy^PuAC                    B,F,J,K,N,O,Q,R,U,X                             
                     MnnI                GTPyPuAC                                                                     
 HindIII                                 A^AGCTT                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X,Y                 
                     Asp52I              AAGCTT                                                                       
                     Asp3065I            AAGCTT                                                                       
                     BbrI                A^AGCTT                                                                      
                     BbrAI               AAGCTT                                                                       
                     BpeI                AAGCTT                                                                       
                     BseHI               AAGCTT                                                                       
                     BsmGII              AAGCTT                                                                       
                     BspKT8I             A^AGCTT                                                                      
                     BspLAIII            A^AGCTT                                                                      
                     Bst170II            AAGCTT                                                                       
                     BstFI               A^AGCTT                                                                      
                     BstZ1II             AAGCTT                                                                       
                     Cfr32I              AAGCTT                                                                       
                     ChuEI               AAGCTT                                                                       
                     Csp231I             AAGCTT                                                                       
                     Eco65I              AAGCTT                                                                       
                     Eco98I              AAGCTT                                                                       
                     Eco188I             AAGCTT                                                                       
                     Eco231I             AAGCTT                                                                       
                     EcoVIII             A^AGCTT                                                                      
                     Hin5III             AAGCTT                                                                       
                     Hin173I             AAGCTT                                                                       
                     Hin1076III          AAGCTT                                                                       
                     HinJCII             AAGCTT                                                                       
                     HinSAFI             AAGCTT                                                                       
                     HinbIII             AAGCTT                                                                       
                     HinfII              AAGCTT                                                                       
                     HsuI                A^AGCTT                                                                      
                     LlaCI               A^AGCTT                                                                      
                     MkiI                AAGCTT                                                                       
                     SpaPIV              AAGCTT                                                                       
                     SsbI                A^AGCTT                                                                      
                     Uba83I              AAGCTT                                                                       
                     Uba1164II           AAGCTT                                                                       
                     Uba1219I            AAGCTT                                                                       
                     Uba1435I            AAGCTT                                                                       
                     UbaN17I             AAGCTT                                                                       
 HinfI                                   G^ANTC                 2     B,C,F,I,J,K,M,N,O,Q,R,U,V,X,Y                   
                     CviBI               G^ANTC                                                                       
                     CviCI               GANTC                                                                        
                     CviDI               GANTC                                                                        
                     CviEI               GANTC                                                                        
                     CviFI               GANTC                                                                        
                     CviGI               GANTC                                                                        
                     FnuAI               G^ANTC                                                                       
                     HhaII               G^ANTC                                                                       
                     HpyIV               GANTC                                                                        
                     HpyAIV              GANTC                                                                        
                     Hpy87AI             GANTC                                                                        
                     HpyF2II             GANTC                                                                        
                     HpyF10II            GANTC                                                                        
                     HpyF18I             GANTC                                                                        
                     HpyF38I             GANTC                                                                        
                     HpyF44I             GANTC                                                                        
                     HpyF55II            GANTC                                                                        
                     HpyF60I             GANTC                                                                        
                     HpyF71III           GANTC                                                                        
                     HpyF72III           GANTC                                                                        
                     NcaI                GANTC                                                                        
                     NovII               GANTC                                                                        
                     NsiHI               GANTC                                                                        
                     SscL1I              G^ANTC                                                                       
 HinfIII                                 CGAAT                  3                                                     
                     HineI               CGAAT                                                                        
 HpaI                                    GTT^AAC                2     B,C,I,J,K,M,N,Q,R,S,V,X                         
                     BsaKI               GTTAAC                                                                       
                     BseII               GTTAAC                                                                       
                     BstEZ359I           GTT^AAC                                                                      
                     BstHPI              GTT^AAC                                                                      
                     KspAI               GTT^AAC                      F                                               
                     MwhI                GTTAAC                                                                       
                     SsrI                GTT^AAC                                                                      
                     Uba1408II           GTTAAC                                                                       
 HpaII                                   C^CGG                  2     F,I,N,Q,R,S,U,V,X                               
                     Asp748I             CCGG                                                                         
                     Bco27I              C^CGG                                                                        
                     BsaZI               CCGG                                                                         
                     BshMI               CCGG                                                                         
                     BsiSI               C^CGG                        C,Y                                             
                     Bsp5I               CCGG                                                                         
                     Bsp47I              CCGG                                                                         
                     Bsp48I              CCGG                                                                         
                     Bsp116I             CCGG                                                                         
                     Bsp1591II           CCGG                                                                         
                     Bst40I              C^CGG                                                                        
                     Bsu1192I            CCGG                                                                         
                     BsuFI               C^CGG                                                                        
                     CboI                C^CGG                                                                        
                     CceI                CCGG                                                                         
                     FinII               CCGG                                                                         
                     FpsJI               C^CGG                                                                        
                     HapII               C^CGG                        B,K                                             
                     Hin2I               C^CGG                                                                        
                     Hin5I               CCGG                                                                         
                     HpyVIII             CCGG                                                                         
                     Hpy299IX            CCGG                                                                         
                     HpyF43I             CCGG                                                                         
                     MniII               CCGG                                                                         
                     MnoI                C^CGG                                                                        
                     MspI                C^CGG                        F,I,J,K,N,Q,R,S,U,V,X                           
                     Msp199I             CCGG                                                                         
                     NflHII              C^CGG                                                                        
                     Pde137I             C^CGG                                                                        
                     Pme35I              CCGG                                                                         
                     SecII               CCGG                                                                         
                     SfaGUI              CCGG                                                                         
                     SmoLI               C^CGG                                                                        
                     Sth134I             C^CGG                                                                        
                     Sth302II            CC^GG                                                                        
                     Uba1128I            CCGG                                                                         
                     Uba1141I            CCGG                                                                         
                     Uba1267I            CCGG                                                                         
                     Uba1338I            CCGG                                                                         
                     Uba1355I            CCGG                                                                         
                     Uba1439I            CCGG                                                                         
                     UbaN13I             CCGG                                                                         
 HphI                                    GGTGA(8/7)             2     F,N                                             
                     AsuHPI              GGTGA(8/7)                   I,V                                             
                     NgoBVIII            GGTGA                                                                        
                     NgoDVIII            GGTGA                                                                        
                     NgoJVIII            GGTGA                                                                        
                     NgoMVIII            GGTGA                                                                        
                     SspD5I              GGTGA(8/8)                                                                   
 Hpy99I                                  CG(A/T)CG^             2     N                                               
                     HpyF61III           CG(A/T)CG                                                                    
 Hpy99XIII                               GCCTA                  2                                                     
 Hpy99XIV-mut1                           GGWCNA                 2                                                     
 Hpy99XIV                                GGWTAA                 2                                                     
 Hpy99XVI                                PuTAPyNNNNNPuTAPy      1                                                     
 Hpy99XXII                               TCANNNNNNTRG           2                                                     
 Hpy178III                               TC^NNGA                2                                                     
                     Hpy188III           TC^NNGA                      N                                               
                     HpyCH4VI            TCNNGA                                                                       
                     HpyF16II            TCNNGA                                                                       
                     HpyF19III           TCNNGA                                                                       
                     HpyF22III           TCNNGA                                                                       
                     HpyF44IV            TCNNGA                                                                       
                     HpyGI               TCNNGA                                                                       
                     HpyNSH57II          TCNNGA                                                                       
 Hpy188I                                 TCN^GA                 2     N                                               
                     Hpy166I             TCNGA                                                                        
                     HpyF11II            TCNGA                                                                        
                     HpyF12II            TCNGA                                                                        
                     HpyF15II            TCNGA                                                                        
                     HpyF17I             TCNGA                                                                        
                     HpyF19II            TCNGA                                                                        
                     HpyF27II            TCNGA                                                                        
                     HpyF28I             TCNGA                                                                        
                     HpyF33I             TCNGA                                                                        
                     HpyF42III           TCNGA                                                                        
                     HpyF46IV            TCNGA                                                                        
                     HpyF50II            TCNGA                                                                        
                     HpyF61I             TCNGA                                                                        
                     HpyF64III           TCNGA                                                                        
 HpyAXIV                                 GCGTA                  2                                                     
 HpyAXVI-mut1                            CRTTAA                 2                                                     
 HpyAXVI-mut2                            CRTCNA                 2                                                     
 HpyAXVIII                               GGANNAG                1                                                     
 HpyUM032XIII                            CPyANNNNNNNTPuG        2                                                     
 HpyUM032XIV                             GAAAG                  2                                                     
 Jma19592I                               GTATNAC                2                                                     
 KpnI                                    GGTAC^C                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X,Y                 
                     Acc65I              G^GTACC                      F,I,N,R,V                                       
                     AhaB8I              G^GTACC                                                                      
                     Asp718I             G^GTACC                      M                                               
                     BspJ106I            GGTACC                                                                       
                     Eco149I             GGTACC                                                                       
                     Esp19I              GGTACC                                                                       
                     KpnK14I             GGTACC                                                                       
                     MvsI                GGTACC                                                                       
                     MvsAI               GGTACC                                                                       
                     MvsBI               GGTACC                                                                       
                     MvsCI               GGTACC                                                                       
                     MvsDI               GGTACC                                                                       
                     MvsEI               GGTACC                                                                       
                     NmiI                GGTACC                                                                       
                     Sau10I              GGTACC                                                                       
                     SthI                G^GTACC                                                                      
                     SthAI               GGTACC                                                                       
                     SthBI               GGTACC                                                                       
                     SthCI               GGTACC                                                                       
                     SthDI               GGTACC                                                                       
                     SthEI               GGTACC                                                                       
                     SthFI               GGTACC                                                                       
                     SthGI               GGTACC                                                                       
                     SthHI               GGTACC                                                                       
                     SthJI               GGTACC                                                                       
                     SthKI               GGTACC                                                                       
                     SthLI               GGTACC                                                                       
                     SthMI               GGTACC                                                                       
                     SthNI               GGTACC                                                                       
                     Uba76I              GGTACC                                                                       
                     Uba85I              GGTACC                                                                       
                     Uba86I              GGTACC                                                                       
                     Uba87I              GGTACC                                                                       
                     Uba1201I            GGTACC                                                                       
 KpnAI                                   GAANNNNNNTGCC          1                                                     
 KpnBI                                   CAAANNNNNNRTCA         1                                                     
 KpnNIH30III                             GTTCNAC                2                                                     
 Ksp632I                                 CTCTTC(1/4)            2                                                     
                     Bco5I               CTCTTC(1/4)                                                                  
                     Bco116I             CTCTTC(1/4)                                                                  
                     BcoKI               CTCTTC(1/4)                                                                  
                     BcoSI               CTCTTC                                                                       
                     BcrAI               CTCTTC                                                                       
                     BseZI               CTCTTC(1/4)                                                                  
                     BsrEI               CTCTTC                                                                       
                     Bst6I               CTCTTC(1/4)                  I,V                                             
                     Bst158I             CTCTTC                                                                       
                     Bsu6I               CTCTTC(1/4)                                                                  
                     CatHI               CTCTTC(1/4)                                                                  
                     Eam1104I            CTCTTC(1/4)                  F                                               
                     EarI                CTCTTC(1/4)                  N                                               
                     TdeII               CTCTTC                                                                       
                     Uba1192I            CTCTTC                                                                       
                     Uba1276I            CTCTTC                                                                       
                     UbaN7I              CTCTTC                                                                       
                     VpaKutEI            CTCTTC                                                                       
                     VpaKutFI            CTCTTC                                                                       
                     VpaO5I              CTCTTC                                                                       
 LlaBIII                                 TNAGCC                 1                                                     
 LlaGI                                   CTNGAYG                1                                                     
 Lmo911II                                TAGRAG                 2                                                     
                     Lmo11AII            TAGRAG                                                                       
 MaeI                                    C^TAG                  2     M                                               
                     BfaI                C^TAG                        N                                               
                     CchI                C^TAG                                                                        
                     FgoI                C^TAG                                                                        
                     FisI                CTAG                                                                         
                     FspBI               C^TAG                        F                                               
                     MjaI                CTAG                                                                         
                     MthFI               CTAG                                                                         
                     MthZI               C^TAG                                                                        
                     RmaI                C^TAG                                                                        
                     Rma485I             CTAG                                                                         
                     Rma486I             CTAG                                                                         
                     Rma490I             CTAG                                                                         
                     Rma495I             CTAG                                                                         
                     Rma496I             CTAG                                                                         
                     Rma497I             CTAG                                                                         
                     Rma500I             CTAG                                                                         
                     Rma501I             CTAG                                                                         
                     Rma503I             CTAG                                                                         
                     Rma506I             CTAG                                                                         
                     Rma509I             CTAG                                                                         
                     Rma510I             CTAG                                                                         
                     Rma515I             CTAG                                                                         
                     Rma516I             CTAG                                                                         
                     Rma517I             CTAG                                                                         
                     Rma518I             CTAG                                                                         
                     Rma519I             CTAG                                                                         
                     Rma522I             CTAG                                                                         
                     UbaN5I              CTAG                                                                         
                     XspI                C^TAG                        K                                               
 MaeII                                   A^CGT                  2     M                                               
                     HpyCH4IV            A^CGT                        N                                               
                     HpyF13III           ACGT                                                                         
                     HpyF35II            ACGT                                                                         
                     HpyF74II            ACGT                                                                         
                     HpySE526I           A^CGT                        I                                               
                     TaiI                ACGT^                        F                                               
                     TscI                ACGT^                                                                        
                     Tsp49I              ACGT^                                                                        
                     TspIDSI             ACGT                                                                         
                     TspWAM8AI           ACGT                                                                         
                     TtmI                ACGT                                                                         
 MaeIII                                  ^GTNAC                 2     M                                               
 MaqI                                    CRTTGAC(21/19)         2                                                     
 MauBI                                   CG^CGCGCG              2     F                                               
 MboI                                    ^GATC                  2     B,C,F,K,N,Q,R,U,X,Y                             
                     Asi256I             G^ATC                                                                        
                     AspMDI              ^GATC                                                                        
                     AsuMBI              GATC                                                                         
                     Bce243I             ^GATC                                                                        
                     Bfi57I              ^GATC                                                                        
                     BfiSHI              GATC                                                                         
                     BfuCI               ^GATC                        N                                               
                     Bme12I              ^GATC                                                                        
                     Bme2494I            GATC                                                                         
                     BsaPI               GATC                                                                         
                     BscFI               ^GATC                                                                        
                     BsmXII              GATC                                                                         
                     BspI                GATC                                                                         
                     Bsp9I               GATC                                                                         
                     Bsp18I              GATC                                                                         
                     Bsp49I              GATC                                                                         
                     Bsp51I              GATC                                                                         
                     Bsp52I              GATC                                                                         
                     Bsp54I              GATC                                                                         
                     Bsp57I              GATC                                                                         
                     Bsp58I              GATC                                                                         
                     Bsp59I              GATC                                                                         
                     Bsp60I              GATC                                                                         
                     Bsp61I              GATC                                                                         
                     Bsp64I              GATC                                                                         
                     Bsp65I              GATC                                                                         
                     Bsp66I              GATC                                                                         
                     Bsp67I              ^GATC                                                                        
                     Bsp72I              GATC                                                                         
                     Bsp74I              GATC                                                                         
                     Bsp76I              GATC                                                                         
                     Bsp91I              GATC                                                                         
                     Bsp105I             ^GATC                                                                        
                     Bsp122I             GATC                                                                         
                     Bsp135I             GATC                                                                         
                     Bsp136I             GATC                                                                         
                     Bsp138I             GATC                                                                         
                     Bsp143I             ^GATC                        F                                               
                     Bsp147I             GATC                                                                         
                     Bsp2095I            ^GATC                                                                        
                     BspAI               ^GATC                                                                        
                     BspFI               ^GATC                                                                        
                     BspJI               ^GATC                                                                        
                     BspJ64I             GATC                                                                         
                     BspKMI              ^GATC                                                                        
                     BspKT6I             GAT^C                                                                        
                     BsrMI               GATC                                                                         
                     BsrPII              GATC                                                                         
                     BssGII              GATC                                                                         
                     BssMI               ^GATC                        V                                               
                     Bst19II             ^GATC                                                                        
                     Bst1274I            GATC                                                                         
                     BstEIII             GATC                                                                         
                     BstENII             ^GATC                                                                        
                     BstKTI              GAT^C                        I                                               
                     BstMBI              ^GATC                        I,V                                             
                     BstXII              GATC                                                                         
                     BtcI                GATC                                                                         
                     Bth84I              GATC                                                                         
                     Bth211I             GATC                                                                         
                     Bth213I             GATC                                                                         
                     Bth221I             GATC                                                                         
                     Bth945I             GATC                                                                         
                     Bth1140I            GATC                                                                         
                     Bth1141I            GATC                                                                         
                     Bth1786I            GATC                                                                         
                     Bth1997I            GATC                                                                         
                     BthCanI             GATC                                                                         
                     BtkII               ^GATC                                                                        
                     Btu33I              GATC                                                                         
                     Btu34I              GATC                                                                         
                     Btu36I              GATC                                                                         
                     Btu37I              GATC                                                                         
                     Btu39I              GATC                                                                         
                     Btu41I              GATC                                                                         
                     CacI                ^GATC                                                                        
                     CcoP31I             GATC                                                                         
                     CcoP76I             GATC                                                                         
                     CcoP84I             GATC                                                                         
                     CcoP95II            GATC                                                                         
                     CcoP219I            GATC                                                                         
                     CcyI                ^GATC                                                                        
                     CdiCD6II            GATC                                                                         
                     ChaI                GATC^                                                                        
                     Cin1467I            GATC                                                                         
                     CjeP338I            GATC                                                                         
                     CpaPI               GATC                                                                         
                     CpfI                ^GATC                                                                        
                     CpfAI               GATC                                                                         
                     Csp5I               GATC                                                                         
                     Cte1179I            GATC                                                                         
                     Cte1180I            GATC                                                                         
                     CtyI                GATC                                                                         
                     CviAI               ^GATC                                                                        
                     CviHI               GATC                                                                         
                     DpnII               ^GATC                        N                                               
                     EsaLHCI             GATC                                                                         
                     FnuAII              GATC                                                                         
                     FnuCI               ^GATC                                                                        
                     FnuEI               ^GATC                                                                        
                     Gst1588II           GATC                                                                         
                     HacI                ^GATC                                                                        
                     HpyAIII             GATC                                                                         
                     HpyHPK5II           GATC                                                                         
                     Kzo9I               ^GATC                        I                                               
                     LlaAI               ^GATC                                                                        
                     LlaDCHI             GATC                                                                         
                     LlaKR2I             GATC                                                                         
                     Lsp1109II           GATC                                                                         
                     Mel3JI              GATC                                                                         
                     Mel5JI              GATC                                                                         
                     Mel7JI              GATC                                                                         
                     Mel4OI              GATC                                                                         
                     Mel5OI              GATC                                                                         
                     Mel2TI              GATC                                                                         
                     Mel5TI              GATC                                                                         
                     MeuI                GATC                                                                         
                     MgoI                ^GATC                                                                        
                     MjaIII              GATC                                                                         
                     MkrAI               ^GATC                                                                        
                     MmeII               GATC                                                                         
                     Mmu5I               GATC                                                                         
                     MmuP2I              GATC                                                                         
                     MnoIII              GATC                                                                         
                     MosI                GATC                                                                         
                     Msp67II             GATC                                                                         
                     MspBI               GATC                                                                         
                     MthI                GATC                                                                         
                     Mth1047I            GATC                                                                         
                     MthAI               GATC                                                                         
                     NciAI               GATC                                                                         
                     NdeII               ^GATC                        J,M                                             
                     NflI                GATC                                                                         
                     NflAII              GATC                                                                         
                     NflBI               GATC                                                                         
                     NlaII               ^GATC                                                                        
                     NlaDI               GATC                                                                         
                     NmeCI               ^GATC                                                                        
                     NphI                ^GATC                                                                        
                     NsiAI               GATC                                                                         
                     NspAI               GATC                                                                         
                     NsuI                GATC                                                                         
                     PbrTI               ^GATC                                                                        
                     Pei9403I            GATC                                                                         
                     PfaI                GATC                                                                         
                     Pph288I             GATC                                                                         
                     RalF40I             ^GATC                                                                        
                     Rlu1I               GATC                                                                         
                     SalAI               GATC                                                                         
                     SalHI               GATC                                                                         
                     Sau15I              GATC                                                                         
                     Sau6782I            GATC                                                                         
                     Sau3AI              ^GATC                        C,J,K,M,N,R,S,U,X                               
                     SauCI               GATC                                                                         
                     SauDI               GATC                                                                         
                     SauEI               GATC                                                                         
                     SauFI               GATC                                                                         
                     SauGI               GATC                                                                         
                     SauMI               ^GATC                                                                        
                     SinMI               GATC                                                                         
                     SmiMBI              GATC                                                                         
                     SsiAI               ^GATC                                                                        
                     SsiBI               ^GATC                                                                        
                     Ssu211I             GATC                                                                         
                     Ssu212I             GATC                                                                         
                     Ssu220I             GATC                                                                         
                     R1.Ssu2479I         GATC                                                                         
                     R2.Ssu2479I         GATC                                                                         
                     R1.Ssu4109I         GATC                                                                         
                     R2.Ssu4109I         GATC                                                                         
                     R1.Ssu4961I         GATC                                                                         
                     R2.Ssu4961I         GATC                                                                         
                     R1.Ssu8074I         GATC                                                                         
                     R2.Ssu8074I         GATC                                                                         
                     R1.Ssu11318I        GATC                                                                         
                     R2.Ssu11318I        GATC                                                                         
                     R1.SsuDAT1I         GATC                                                                         
                     R2.SsuDAT1I         GATC                                                                         
                     Sth368I             ^GATC                                                                        
                     TrsKTI              GATC                                                                         
                     TrsSI               GATC                                                                         
                     TrsTI               GATC                                                                         
                     TruII               GATC                                                                         
                     Tsp133I             GATC                                                                         
                     Uba4I               GATC                                                                         
                     Uba59I              GATC                                                                         
                     Uba1101I            GATC                                                                         
                     Uba1177I            GATC                                                                         
                     Uba1182I            GATC                                                                         
                     Uba1183I            GATC                                                                         
                     Uba1204I            GATC                                                                         
                     Uba1259I            GATC                                                                         
                     Uba1317I            GATC                                                                         
                     Uba1323I            GATC                                                                         
                     Uba1366I            GATC                                                                         
                     UbaN1I              GATC                                                                         
                     Vha44I              GATC                                                                         
 MboII                                   GAAGA(8/7)             2     F,I,J,K,N,Q,R,V,X                               
                     HpyII               GAAGA                                                                        
                     Hpy178II            GAAGA                                                                        
                     HpyAII              GAAGA                                                                        
                     NcuI                GAAGA(8/7)                                                                   
                     TceI                GAAGA                                                                        
 McrI                                    CGPuPy^CG              2                                                     
                     BsaOI               CGPuPy^CG                                                                    
                     BscNI               CGPuPyCG                                                                     
                     Bsh1285I            CGPuPy^CG                    F                                               
                     BsiEI               CGPuPy^CG                    N                                               
                     BstMCI              CGPuPy^CG                    I,V                                             
                     BstZ5I              CGPuPyCG                                                                     
                     Uba1303I            CGPuPyCG                                                                     
 MfeI                                    C^AATTG                2     I,N                                             
                     MunI                C^AATTG                      F,K,M                                           
 MjaIV                                   GTNNAC                 2                                                     
                     Hpy8I               GTN^NAC                      F                                               
                     Hpy166II            GTN^NAC                      N                                               
                     HpyBII              GTN^NAC                                                                      
                     HpyF7III            GTNNAC                                                                       
                     HpyF14II            GTNNAC                                                                       
                     HpyF46III           GTNNAC                                                                       
                     HpyF49III           GTNNAC                                                                       
                     HpyF50I             GTNNAC                                                                       
 MkaDII                                  GAGAYGT                2                                                     
 MluI                                    A^CGCGT                2     B,F,I,J,K,M,N,O,Q,R,V,X                         
                     ApeI                ACGCGT                                                                       
                     Bbi24I              A^CGCGT                                                                      
                     BstZ9I              ACGCGT                                                                       
                     Uba6I               ACGCGT                                                                       
 MmeI                                    TCCRAC(20/18)          2     N,X                                             
 MmyCI                                   TGAG                   3                                                     
 MnlI                                    CCTC(7/6)              2     F,I,N,Q,V,X                                     
                     Hpy166III           CCTC                                                                         
 MseI                                    T^TAA                  2     B,N                                             
                     SaqAI               T^TAA                        F                                               
                     Tru1I               T^TAA                        F                                               
                     Tru9I               T^TAA                        I,M,R,V                                         
 MslI                                    CAPyNN^NNPuTG          2     N                                               
                     RseI                CAPyNN^NNPuTG                F                                               
                     SmiMI               CAPyNN^NNPuTG                I,V                                             
 MstI                                    TGC^GCA                2                                                     
                     AcaIII              TGCGCA                                                                       
                     Acc16I              TGC^GCA                      I,V                                             
                     AosI                TGC^GCA                                                                      
                     ApcTR183I           TGCGCA                                                                       
                     AviII               TGC^GCA                                                                      
                     Bco6I               TGCGCA                                                                       
                     BsaTI               TGCGCA                                                                       
                     ClcII               TGCGCA                                                                       
                     CliII               TGCGCA                                                                       
                     FdiII               TGC^GCA                                                                      
                     FspI                TGC^GCA                      J,N                                             
                     GspAII              TGCGCA                                                                       
                     NsbI                TGC^GCA                      F,K                                             
                     NspHIII             TGCGCA                                                                       
                     NspLI               TGCGCA                                                                       
                     NspMI               TGCGCA                                                                       
                     PamI                TGC^GCA                                                                      
                     Pun14627I           TGC^GCA                                                                      
 Mva1261III                              CTANNNNNNRTTC          1                                                     
 MwoI                                    GCNNNNN^NNGC           2     N                                               
                     Bce4I               GCNNNNN^NNGC                                                                 
                     Bce1247I            GCNNNNNNNGC                                                                  
                     BceCI               GCNNNNN^NNGC                                                                 
                     BspWI               GCNNNNN^NNGC                                                                 
                     BstMWI              GCNNNNN^NNGC                 I                                               
                     HpyF10VI            GCNNNNN^NNGC                 F                                               
 NaeI                                    GCC^GGC                2     C,K,N                                           
                     Afa24RI             GCCGGC                                                                       
                     AmeII               GCCGGC                                                                       
                     AniMI               GCCGGC                                                                       
                     ApeAI               GCCGGC                                                                       
                     AprI                GCCGGC                                                                       
                     BheI                GCCGGC                                                                       
                     CcoI                GCC^GGC                                                                      
                     Eco56I              G^CCGGC                                                                      
                     Esp5I               GCCGGC                                                                       
                     MauAI               GCCGGC                                                                       
                     MisI                GCCGGC                                                                       
                     Mlu9273II           GCCGGC                                                                       
                     MroNI               G^CCGGC                      I,V                                             
                     NbaI                GCCGGC                                                                       
                     NbrI                GCCGGC                                                                       
                     NgoAIV              G^CCGGC                                                                      
                     NgoMIV              G^CCGGC                      N                                               
                     NmuI                GCCGGC                                                                       
                     NmuFI               GCCGGC                                                                       
                     NspWI               GCCGGC                                                                       
                     NtaSII              GCCGGC                                                                       
                     PdiI                GCC^GGC                      F                                               
                     PglI                GCCGGC                                                                       
                     Psp61I              GCCGGC                                                                       
                     RluI                GCCGGC                                                                       
                     RrhJ1I              GCC^GGC                                                                      
                     SacAI               GCCGGC                                                                       
                     SalCI               GCCGGC                                                                       
                     SaoI                GCCGGC                                                                       
                     SauAI               GCCGGC                                                                       
                     SauBMKI             GCC^GGC                                                                      
                     SauHPI              GCC^GGC                                                                      
                     SauLPI              GCC^GGC                                                                      
                     SauNI               GCC^GGC                                                                      
                     SauSI               GCC^GGC                                                                      
                     SceIII              G^CCGGC                                                                      
                     SghWII              GCCGGC                                                                       
                     SkaI                GCCGGC                                                                       
                     Slu1777I            GCC^GGC                                                                      
                     Smo40529I           GCCGGC                                                                       
                     SrlI                G^CCGGC                                                                      
                     Srl77DI             GCCGGC                                                                       
                     SspCI               GCC^GGC                                                                      
                     Uba1122I            GCCGGC                                                                       
 NarI                                    GG^CGCC                2     J,M,N,Q,R,X                                     
                     BbeI                GGCGC^C                                                                      
                     BbeAI               GGCGCC                                                                       
                     R1.BbrUI            GGCGCC                                                                       
                     BinSII              GGCGC^C                                                                      
                     DinI                GGC^GCC                      V                                               
                     Eco78I              GGC^GCC                                                                      
                     EgeI                GGC^GCC                      I                                               
                     EheI                GGC^GCC                      F                                               
                     KasI                G^GCGCC                      N                                               
                     McaAI               GGCGCC                                                                       
                     MchI                GG^CGCC                                                                      
                     Mly113I             GG^CGCC                      I                                               
                     MsaI                GGCGCC                                                                       
                     NamI                GGCGCC                                                                       
                     NdaI                GG^CGCC                                                                      
                     NunII               GG^CGCC                                                                      
                     PatAI               GGCGCC                                                                       
                     PluTI               GGCGC^C                      N                                               
                     PmnI                GGCGCC                                                                       
                     SfoI                GGC^GCC                      N                                               
                     SseAI               GG^CGCC                                                                      
                     SspDI               G^GCGCC                      F                                               
 NcoI                                    C^CATGG                2     B,C,F,J,K,M,N,O,Q,R,S,U,X,Y                     
                     AteI                CCATGG                                                                       
                     Bse19I              CCATGG                                                                       
                     Bsp19I              C^CATGG                      I,V                                             
                     NspSAIII            CCATGG                                                                       
                     Pam7686I            C^CATGG                                                                      
 NdeI                                    CA^TATG                2     B,F,J,K,M,N,Q,R,S,U,X                           
                     FauNDI              CA^TATG                      I,V                                             
                     PfaAII              CA^TATG                                                                      
                     TrsKTIII            CATATG                                                                       
 NgoAV                                   GCANNNNNNNNTGC         1                                                     
 NgoAVII                                 GCCGC(7/7)             2                                                     
 NgoAVIII                                (12/14)GACNNNNNTGA(13/11)2                                                     
 NgoAX                                   CCACC                  3                                                     
                     NgoMX               CCACC                                                                        
 NhaXI                                   CAAGRAG                2                                                     
 NheI                                    G^CTAGC                2     B,C,F,J,K,M,N,O,Q,R,S,U,X                       
                     AceII               GCTAG^C                                                                      
                     AsuNHI              G^CTAGC                      I,V                                             
                     BmtI                GCTAG^C                      I,N,V                                           
                     BspOI               GCTAG^C                      F                                               
                     LlaG2I              G^CTAGC                                                                      
                     PstNHI              G^CTAGC                                                                      
 NlaIII                                  CATG^                  2     N                                               
                     CviAII              C^ATG                        N                                               
                     FaeI                CATG^                        I                                               
                     FatI                ^CATG                        I,N                                             
                     FatII               CATG                                                                         
                     Hin1II              CATG^                        F                                               
                     Hin8II              CATG                                                                         
                     HpyCH4I             CATG^                                                                        
                     Hsp92II             CATG^                        R                                               
 NlaIV                                   GGN^NCC                2     N                                               
                     AspNI               GGN^NCC                                                                      
                     BcrI                GGNNCC                                                                       
                     BmiI                GGN^NCC                      V                                               
                     BsaEI               GGNNCC                                                                       
                     BscBI               GGN^NCC                                                                      
                     Bsp29I              GGNNCC                                                                       
                     BspLI               GGN^NCC                      F                                               
                     BssI                GGNNCC                                                                       
                     HpyF44II            GGNNCC                                                                       
                     HpyF73I             GGNNCC                                                                       
                     NgoBV               GGNNCC                                                                       
                     NgoGV               GGNNCC                                                                       
                     NgoMV               GGNNCC                                                                       
                     PspN4I              GGN^NCC                      I                                               
                     Rlu3I               GGNNCC                                                                       
                     Uba1305I            GGNNCC                                                                       
                     Uba1445I            GGNNCC                                                                       
 NlaCI                                   CATCAC(19/17)          2                                                     
 NmeAIII                                 GCCGAG(21/19)          2     N                                               
 NotI                                    GC^GGCCGC              2     B,C,F,J,K,M,N,O,Q,R,S,U,X                       
                     CciNI               GC^GGCCGC                    I,V                                             
                     CsiBI               GC^GGCCGC                                                                    
                     CspBI               GC^GGCCGC                                                                    
                     MchAI               GC^GGCCGC                                                                    
 NruI                                    TCG^CGA                2     B,C,I,J,K,M,N,Q,R,X                             
                     AmaSI               TCGCGA                                                                       
                     Bsp68I              TCG^CGA                      F                                               
                     BtuMI               TCG^CGA                      V                                               
                     Mlu9273I            TCGCGA                                                                       
                     MluB2I              TCG^CGA                                                                      
                     PspDI               TCGCGA                                                                       
                     RruI                TCG^CGA                      F                                               
                     SalDI               TCGCGA                                                                       
                     Sbo13I              TCG^CGA                                                                      
                     Sna3286I            TCGCGA                                                                       
                     SpoI                TCG^CGA                                                                      
                     Uba1117I            TCGCGA                                                                       
                     Uba1386I            TCGCGA                                                                       
                     VchO70I             TCGCGA                                                                       
 NspI                                    PuCATG^Py              2     N                                               
                     AvoI                PuCATG^Py                                                                    
                     BstNSI              PuCATG^Py                    I,V                                             
                     Lsp1270I            PuCATGPy                                                                     
                     NspHI               PuCATG^Py                                                                    
                     PauAI               PuCATG^Py                                                                    
                     PunAII              PuCATG^Py                                                                    
                     XceI                PuCATG^Py                    F                                               
 NspBII                                  C(A/C)G^C(G/T)G        2                                                     
                     MspA1I              C(A/C)G^C(G/T)G              I,N,R,V                                         
 OliI                                    CACNN^NNGTG            2     F                                               
                     AleI                CACNN^NNGTG                  N                                               
 PacI                                    TTAAT^TAA              2     F,N,O                                           
 PasI                                    CC^C(A/T)GGG           2     F                                               
 Pdi8503III                              CCGGNAG                2                                                     
 PenI                                    GCAGT                  2                                                     
                     SmoLII              GCAGT                                                                        
 Pfl1108I                                TCGTAG                 2                                                     
 PflMI                                   CCANNNN^NTGG           2     N                                               
                     AccB7I              CCANNNN^NTGG                 I,V                                             
                     AcpII               CCANNNN^NTGG                                                                 
                     Asp10HII            CCANNNN^NTGG                                                                 
                     BasI                CCANNNN^NTGG                                                                 
                     Esp1396I            CCANNNN^NTGG                                                                 
                     PflBI               CCANNNN^NTGG                                                                 
                     Van91I              CCANNNN^NTGG                 F,K                                             
 PfoI                                    T^CCNGGA               2     F                                               
 PleI                                    GAGTC(4/5)             2     N                                               
                     BsmEI               GAGTC                                                                        
                     BspD6I              GACTC(4/6)                                                                   
                     MlyI                GAGTC(5/5)                   N                                               
                     PpsI                GAGTC(4/5)                   I                                               
                     SchI                GAGTC(5/5)                   F                                               
 PmaCI                                   CAC^GTG                2     K                                               
                     AcvI                CAC^GTG                      Q,X                                             
                     BbrPI               CAC^GTG                      M                                               
                     BcoAI               CAC^GTG                                                                      
                     Bsp87I              CACGTG                                                                       
                     Eco72I              CAC^GTG                      F                                               
                     Pgl34I              CACGTG                                                                       
                     PmlI                CAC^GTG                      N                                               
                     PshCI               CACGTG                                                                       
                     PshDI               CACGTG                                                                       
                     Psp38I              CACGTG                                                                       
                     PspBI               CACGTG                                                                       
                     PspCI               CAC^GTG                      I,V                                             
                     VpaK3AI             CACGTG                                                                       
                     VpaK3BI             CACGTG                                                                       
 PmeI                                    GTTT^AAAC              2     N                                               
                     MssI                GTTT^AAAC                    F                                               
 Pme5II                                  GACGAG                 2                                                     
 PmeS132I                                GACGAG                 2                                                     
 PpiI                                    (7/12)GAACNNNNNCTC(13/8)2                                                     
 PpuMI                                   PuG^G(A/T)CCPy         2     N                                               
                     AspBHII             PuG^G(A/T)CCPy                                                               
                     Mlu1106I            PuGG(A/T)CCPy                                                                
                     Pfl27I              PuG^G(A/T)CCPy                                                               
                     PpuXI               PuG^G(A/T)CCPy                                                               
                     Psp5II              PuG^G(A/T)CCPy               F                                               
                     PspPPI              PuG^G(A/T)CCPy               I                                               
 PshAI                                   GACNN^NNGTC            2     K,N                                             
                     BoxI                GACNN^NNGTC                  F                                               
                     BstPAI              GACNN^NNGTC                  I,V                                             
 PsiI                                    TTA^TAA                2     I,N                                             
                     AanI                TTA^TAA                      F                                               
 PspOMII                                 CGCCCAR(20/18)         2                                                     
 PspPRI                                  CCYCAG(15/13)          2                                                     
 PspXI                                   (A/C/G)C^TCGAG(C/G/T)  2     I,N                                             
 PsrI                                    (7/12)GAACNNNNNNTAC(12/7)2     I                                               
 PstI                                    CTGCA^G                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X                   
                     AinI                CTGCAG                                                                       
                     AjoI                CTGCA^G                                                                      
                     Ali2882I            CTGCAG                                                                       
                     AliAJI              CTGCA^G                                                                      
                     ApiI                CTGCA^G                                                                      
                     AplI                CTGCA^G                                                                      
                     Asp36I              CTGCAG                                                                       
                     Asp708I             CTGCAG                                                                       
                     Asp713I             CTGCA^G                                                                      
                     AspTI               CTGCAG                                                                       
                     BbiI                CTGCAG                                                                       
                     BbrUIII             CTGCAG                                                                       
                     Bce170I             CTGCAG                                                                       
                     BloHII              CTGCA^G                                                                      
                     BloHIII             CTGCAG                                                                       
                     BmeBI               CTGCAG                                                                       
                     BsaNII              CTGCAG                                                                       
                     BsaQI               CTGCAG                                                                       
                     BscDI               CTGCAG                                                                       
                     Bsp17I              CTGCAG                                                                       
                     Bsp43I              CTGCAG                                                                       
                     Bsp63I              CTGCA^G                                                                      
                     Bsp78I              CTGCAG                                                                       
                     Bsp81I              CTGCAG                                                                       
                     Bsp93I              CTGCAG                                                                       
                     Bsp107I             CTGCAG                                                                       
                     Bsp108I             CTGCAG                                                                       
                     Bsp268I             CTGCAG                                                                       
                     BspBI               CTGCA^G                                                                      
                     BspMAI              CTGCA^G                                                                      
                     BspS122I            CTGCAG                                                                       
                     BsuBI               CTGCA^G                                                                      
                     Bth9411I            CTGCAG                                                                       
                     CauIII              CTGCAG                                                                       
                     CflI                CTGCA^G                                                                      
                     CfrA4I              CTGCA^G                                                                      
                     CfuII               CTGCA^G                                                                      
                     ClcI                CTGCAG                                                                       
                     CstI                CTGCA^G                                                                      
                     EaePI               CTGCAG                                                                       
                     Ecl77I              CTGCAG                                                                       
                     Ecl133I             CTGCAG                                                                       
                     Ecl593I             CTGCAG                                                                       
                     Ecl37kI             CTGCA^G                                                                      
                     Ecl699kI            CTGCAG                                                                       
                     Ecl1zI              CTGCAG                                                                       
                     Ecl2zI              CTGCA^G                                                                      
                     Eco48I              CTGCAG                                                                       
                     Eco49I              CTGCAG                                                                       
                     Eco83I              CTGCAG                                                                       
                     Eco161I             CTGCAG                                                                       
                     Eco167I             CTGCAG                                                                       
                     Eco260I             CTGCAG                                                                       
                     Eco261I             CTGCAG                                                                       
                     EcoGIII             CTGCAG                                                                       
                     EhoI                CTGCAG                                                                       
                     Esp5II              CTGCAG                                                                       
                     Esp141I             CTGCAG                                                                       
                     GseII               CTGCAG                                                                       
                     HalII               CTGCA^G                                                                      
                     Kpn12I              CTGCAG                                                                       
                     MauI                CTGCAG                                                                       
                     MhaAI               CTGCA^G                                                                      
                     MizI                CTGCAG                                                                       
                     MkrI                CTGCAG                                                                       
                     MmaI                CTGCAG                                                                       
                     Msp11I              CTGCAG                                                                       
                     NasI                CTGCAG                                                                       
                     NgbI                CTGCAG                                                                       
                     NocI                CTGCAG                                                                       
                     Pae8I               CTGCAG                                                                       
                     Pae9I               CTGCAG                                                                       
                     Pae14I              CTGCAG                                                                       
                     Pae15I              CTGCAG                                                                       
                     Pae22I              CTGCAG                                                                       
                     Pae24I              CTGCAG                                                                       
                     Pae25I              CTGCAG                                                                       
                     Pae26I              CTGCAG                                                                       
                     Pae39I              CTGCAG                                                                       
                     Pae40I              CTGCAG                                                                       
                     Pae41I              CTGCAG                                                                       
                     PaeHII              CTGCA^G                                                                      
                     Pfl21I              CTGCA^G                                                                      
                     Pfl37I              CTGCAG                                                                       
                     PmaI                CTGCAG                                                                       
                     Pma44I              CTGCAG                                                                       
                     PmyI                CTGCAG                                                                       
                     Pph2059I            CTGCAG                                                                       
                     Pph2066I            CTGCAG                                                                       
                     PshEI               CTGCAG                                                                       
                     Psp23I              CTGCA^G                                                                      
                     Psp28I              CTGCAG                                                                       
                     Psp46I              CTGCAG                                                                       
                     PspSI               CTGCAG                                                                       
                     Sag16I              CTGCA^G                                                                      
                     Sag23I              CTGCA^G                                                                      
                     Sal13I              CTGCAG                                                                       
                     SalPI               CTGCA^G                                                                      
                     ScoAI               CTGCAG                                                                       
                     SflI                CTGCA^G                                                                      
                     SgiI                CTGCAG                                                                       
                     SkaII               CTGCAG                                                                       
                     SprLI               CTGCAG                                                                       
                     SriI                CTGCAG                                                                       
                     Srl1DI              CTGCAG                                                                       
                     Srl2DI              CTGCAG                                                                       
                     Srl5DI              CTGCA^G                                                                      
                     Srl32DI             CTGCAG                                                                       
                     Ssp12I              CTGCAG                                                                       
                     Sst12I              CTGCA^G                                                                      
                     TspGWII             CTGCAG                                                                       
                     Uba46I              CTGCAG                                                                       
                     Uba71I              CTGCAG                                                                       
                     Uba72I              CTGCAG                                                                       
                     Uba1112I            CTGCAG                                                                       
                     Uba1115I            CTGCAG                                                                       
                     Uba1116I            CTGCAG                                                                       
                     Uba1119I            CTGCAG                                                                       
                     Uba1123I            CTGCAG                                                                       
                     Uba1149I            CTGCAG                                                                       
                     Uba1184I            CTGCAG                                                                       
                     Uba1186I            CTGCAG                                                                       
                     Uba1211I            CTGCAG                                                                       
                     Uba1212I            CTGCAG                                                                       
                     Uba1213I            CTGCAG                                                                       
                     Uba1215I            CTGCAG                                                                       
                     Uba1216I            CTGCAG                                                                       
                     Uba1225I            CTGCAG                                                                       
                     Uba1232I            CTGCAG                                                                       
                     Uba1256I            CTGCAG                                                                       
                     Uba1262I            CTGCAG                                                                       
                     Uba1287I            CTGCAG                                                                       
                     Uba1294II           CTGCAG                                                                       
                     Uba1296I            CTGCAG                                                                       
                     Uba1328I            CTGCAG                                                                       
                     Uba1337I            CTGCAG                                                                       
                     Uba1399I            CTGCAG                                                                       
                     Uba1411I            CTGCAG                                                                       
                     Uba1417I            CTGCAG                                                                       
                     UbaHKBI             CTGCAG                                                                       
                     VchO87I             CTGCAG                                                                       
                     VpaK4AI             CTGCAG                                                                       
                     VpaK29AI            CTGCAG                                                                       
                     VpaK4BI             CTGCAG                                                                       
                     VpaKutGI            CTGCAG                                                                       
                     XcpI                CTGCA^G                                                                      
                     XmaII               CTGCAG                                                                       
                     XorI                CTGCAG                                                                       
                     XorKII              CTGCAG                                                                       
                     XphI                CTGCAG                                                                       
                     XveI                CTGCAG                                                                       
                     YenI                CTGCA^G                                                                      
                     YenAI               CTGCAG                                                                       
                     YenBI               CTGCAG                                                                       
                     YenCI               CTGCAG                                                                       
                     YenDI               CTGCAG                                                                       
                     YenEI               CTGCAG                                                                       
 PstII                                   CTGATG(25/27)          3                                                     
                     PlaDI               CATCAG(21/19)                                                                
                     SsmI                CTGATG                                                                       
 PvuI                                    CGAT^CG                2     B,F,K,M,N,O,Q,R,S,X                             
                     Afa22MI             CGAT^CG                                                                      
                     Afa16RI             CGAT^CG                                                                      
                     BmaI                CGATCG                                                                       
                     BmaAI               CGATCG                                                                       
                     BmaBI               CGATCG                                                                       
                     BmaCI               CGATCG                                                                       
                     BmaDI               CGATCG                                                                       
                     BpvUI               CGAT^CG                                                                      
                     BspCI               CGAT^CG                                                                      
                     BstZ8I              CGATCG                                                                       
                     Cas2I               CGATCG                                                                       
                     Cfr51I              CGATCG                                                                       
                     DrdIII              CGATCG                                                                       
                     EagBI               CGAT^CG                                                                      
                     EclJI               CGATCG                                                                       
                     ErhB9I              CGAT^CG                                                                      
                     Kpl79I              CGATCG                                                                       
                     MvrI                CGAT^CG                                                                      
                     NblI                CGAT^CG                                                                      
                     Ple19I              CGAT^CG                      I                                               
                     PntI                CGATCG                                                                       
                     Psu161I             CGAT^CG                                                                      
                     Pvu84I              CGATCG                                                                       
                     RshI                CGAT^CG                                                                      
                     RspI                CGATCG                                                                       
                     SmaAIII             CGATCG                                                                       
                     SpaPII              CGATCG                                                                       
                     SplAIII             CGATCG                                                                       
                     Uba1129I            CGATCG                                                                       
                     Uba1139I            CGATCG                                                                       
                     Xgl3216I            CGATCG                                                                       
                     Xgl3217I            CGATCG                                                                       
                     Xgl3218I            CGATCG                                                                       
                     Xgl3219I            CGATCG                                                                       
                     Xgl3220I            CGATCG                                                                       
                     XmlI                CGATCG                                                                       
                     XmlAI               CGATCG                                                                       
                     XniI                CGATCG                                                                       
                     XorII               CGAT^CG                                                                      
                     XorKI               CGATCG                                                                       
 PvuII                                   CAG^CTG                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X                   
                     BamGI               CAG^CTG                                                                      
                     BavI                CAG^CTG                                                                      
                     BavAI               CAG^CTG                                                                      
                     BavBI               CAG^CTG                                                                      
                     Bsp153AI            CAG^CTG                                                                      
                     BspM39I             CAG^CTG                                                                      
                     BspO4I              CAG^CTG                                                                      
                     Bth2350I            CAGCTG                                                                       
                     Cfr6I               CAG^CTG                                                                      
                     DmaI                CAG^CTG                                                                      
                     EclI                CAG^CTG                                                                      
                     GspI                CAGCTG                                                                       
                     MziI                CAGCTG                                                                       
                     NmeRI               CAG^CTG                                                                      
                     Pae17kI             CAG^CTG                                                                      
                     Psp3I               CAGCTG                                                                       
                     Psp5I               CAGCTG                                                                       
                     Pun14627II          CAG^CTG                                                                      
                     Pvu84II             CAG^CTG                                                                      
                     PvuHKUI             CAGCTG                                                                       
                     SbaI                CAGCTG                                                                       
                     SciAII              CAGCTG                                                                       
                     SmaAIV              CAGCTG                                                                       
                     Sol3335I            CAGCTG                                                                       
                     SpaPIII             CAGCTG                                                                       
                     SplAIV              CAGCTG                                                                       
                     Uba1227I            CAGCTG                                                                       
                     Uba1245I            CAGCTG                                                                       
                     Uba153AI            CAG^CTG                                                                      
                     UbaM39I             CAG^CTG                                                                      
 RceI                                    CATCGAC(20/18)         2                                                     
 RdeGBI                                  CCGCAG                 2                                                     
 RdeGBII                                 ACCCAG(20/18)          2                                                     
 RdeGBIII                                (9/11)TGRYCA(11/9)     2                                                     
 RflFIII                                 CGCCAG                 2                                                     
 RleAI                                   CCCACA(12/9)           2                                                     
 RpaI                                    GTYGGAG(11/9)          2                                                     
 RpaBI                                   CCCGCAG(20/18)         2                                                     
 RpaB5I                                  CGRGGAC(20/18)         2                                                     
 RpaTI                                   GRTGGAG                2                                                     
 RsaI                                    GT^AC                  2     C,F,I,J,M,N,Q,R,S,V,X,Y                         
                     AfaI                GT^AC                        B,K                                             
                     Asp16HI             GTAC                                                                         
                     Asp17HI             GTAC                                                                         
                     Asp18HI             GTAC                                                                         
                     Asp29HI             GTAC                                                                         
                     CcoP73I             GTAC                                                                         
                     Csp6I               G^TAC                        F                                               
                     CviQI               G^TAC                        N                                               
                     CviRII              G^TAC                                                                        
                     Hpy303I             GTAC                                                                         
                     Hpy312I             GTAC                                                                         
                     Hpy401I             GTAC                                                                         
                     Hpy421I             GTAC                                                                         
                     Hpy423I             GTAC                                                                         
                     Hpy471I             GTAC                                                                         
                     Hpy501I             GTAC                                                                         
                     HpyBI               GT^AC                                                                        
                     HpyF10IV            GTAC                                                                         
                     HpyF13IV            GTAC                                                                         
                     HpyF21II            GTAC                                                                         
                     HpyF31I             GTAC                                                                         
                     HpyF36II            GTAC                                                                         
                     HpyF44V             GTAC                                                                         
                     HpyF52III           GTAC                                                                         
                     HpyF53II            GTAC                                                                         
                     HpyF59II            GTAC                                                                         
                     HpyF65III           GTAC                                                                         
                     HpyF66III           GTAC                                                                         
                     HpyHII              GTAC                                                                         
                     HpyNSH57I           GTAC                                                                         
                     MjaV                GTAC                                                                         
                     PabI                GTA^C                                                                        
                     PlaAII              GT^AC                                                                        
                     RsaNI               G^TAC                        I                                               
 RsrII                                   CG^G(A/T)CCG           2     N,Q,X                                           
                     CpoI                CG^G(A/T)CCG                 F,K                                             
                     CspI                CG^G(A/T)CCG                 R                                               
                     Rsr2I               CG^G(A/T)CCG                 I,V                                             
 SacI                                    GAGCT^C                2     B,F,J,K,M,N,O,Q,R,S,U,X                         
                     BpuAmI              GAG^CTC                                                                      
                     Ecl136II            GAG^CTC                      F                                               
                     Ecl137I             GAGCTC                                                                       
                     EcoICRI             GAG^CTC                      I,R,V                                           
                     Eco53kI             GAG^CTC                      N                                               
                     MxaI                GAG^CTC                                                                      
                     NasSI               GAGCTC                                                                       
                     Pfl18I              GAGCTC                                                                       
                     Psp124BI            GAGCT^C                      I,V                                             
                     ScoI                GAGCTC                                                                       
                     Sgr13350I           GAGCTC                                                                       
                     Ssm4I               GAGCTC                                                                       
                     Ssm5I               GAGCTC                                                                       
                     SstI                GAGCT^C                      C                                               
                     UcoMSI              (7/5)GAGCTC(5/7)                                                             
 SacII                                   CCGC^GG                2     B,J,K,N,O,Q,R,X                                 
                     AosIII              CCGCGG                                                                       
                     Asp32HI             CCGCGG                                                                       
                     BacI                CCGCGG                                                                       
                     Bac465I             CCGCGG                                                                       
                     BloMGI              CCGCGG                                                                       
                     BscTI               CCGCGG                                                                       
                     Bsp12I              CCGCGG                                                                       
                     Cfr37I              CCGCGG                                                                       
                     Cfr41I              CCGCGG                                                                       
                     Cfr42I              CCGC^GG                      F                                               
                     Cfr43I              CCGCGG                                                                       
                     Cfr45II             CCGCGG                                                                       
                     CscI                CCGC^GG                                                                      
                     Cte1I               CCGCGG                                                                       
                     DrdAI               CCGCGG                                                                       
                     DrdBI               CCGCGG                                                                       
                     DrdCI               CCGCGG                                                                       
                     DrdEI               CCGCGG                                                                       
                     DrdFI               CCGCGG                                                                       
                     Dsp1I               CCGCGG                                                                       
                     Eae46I              CCGC^GG                                                                      
                     EccI                CCGCGG                                                                       
                     Ecl1I               CCGCGG                                                                       
                     Ecl28I              CCGCGG                                                                       
                     Ecl37I              CCGCGG                                                                       
                     Eco55I              CCGCGG                                                                       
                     Eco92I              CCGCGG                                                                       
                     Eco96I              CCGCGG                                                                       
                     Eco99I              CCGCGG                                                                       
                     Eco100I             CCGCGG                                                                       
                     Eco104I             CCGCGG                                                                       
                     Eco134I             CCGCGG                                                                       
                     Eco135I             CCGCGG                                                                       
                     Eco151I             CCGCGG                                                                       
                     Eco158I             CCGCGG                                                                       
                     Eco182I             CCGCGG                                                                       
                     Eco196I             CCGCGG                                                                       
                     Eco208I             CCGCGG                                                                       
                     Eco29kI             CCGC^GG                                                                      
                     Ese3I               CCGCGG                                                                       
                     Ese6I               CCGCGG                                                                       
                     FscI                CCGCGG                                                                       
                     GalI                CCGC^GG                                                                      
                     GceI                CCGC^GG                                                                      
                     GceGLI              CCGC^GG                                                                      
                     Kpn19I              CCGCGG                                                                       
                     Kpn378I             CCGC^GG                                                                      
                     KspI                CCGC^GG                      M,S                                             
                     Mpr154I             CCGC^GG                                                                      
                     MraI                CCGCGG                                                                       
                     NgoAIII             CCGC^GG                                                                      
                     NgoDIII             CCGCGG                                                                       
                     NgoGIII             CCGCGG                                                                       
                     NgoJIII             CCGCGG                                                                       
                     NgoKIII             CCGCGG                                                                       
                     NgoMIII             CCGCGG                                                                       
                     NgoPIII             CCGC^GG                                                                      
                     NlaDIII             CCGCGG                                                                       
                     NlaSI               CCGCGG                                                                       
                     Pae7I               CCGCGG                                                                       
                     Pae17I              CCGCGG                                                                       
                     Pae36I              CCGCGG                                                                       
                     Pae42I              CCGCGG                                                                       
                     Pae43I              CCGCGG                                                                       
                     Pae44I              CCGCGG                                                                       
                     PaeAI               CCGC^GG                                                                      
                     PaeQI               CCGC^GG                                                                      
                     Pae5kI              CCGC^GG                                                                      
                     Pae14kI             CCGC^GG                                                                      
                     Pfl1108II           CCGCGG                                                                       
                     RaqI                CCGC^GG                                                                      
                     SaaI                CCGCGG                                                                       
                     SabI                CCGCGG                                                                       
                     SakI                CCGCGG                                                                       
                     SboI                CCGCGG                                                                       
                     SchZI               CCGC^GG                                                                      
                     SenPT14bI           CCGC^GG                                                                      
                     SenpCI              CCGCGG                                                                       
                     SexBI               CCGC^GG                                                                      
                     SexCI               CCGC^GG                                                                      
                     SfrI                CCGCGG                                                                       
                     Sfr303I             CCGC^GG                      I,V                                             
                     Sfr382I             CCGCGG                                                                       
                     SgrBI               CCGC^GG                      C                                               
                     ShyI                CCGCGG                                                                       
                     SpuI                CCGC^GG                                                                      
                     SseII               CCGCGG                                                                       
                     SsmII               CCGCGG                                                                       
                     Ssm5II              CCGCGG                                                                       
                     Ssp1725I            CCGCGG                                                                       
                     SstII               CCGC^GG                                                                      
                     StaI                CCGCGG                                                                       
                     TglI                CCGCGG                                                                       
                     TtoI                CCGCGG                                                                       
                     Uba66I              CCGCGG                                                                       
                     Uba77I              CCGCGG                                                                       
                     Uba90I              CCGCGG                                                                       
                     Uba1093I            CCGCGG                                                                       
                     Uba1095I            CCGCGG                                                                       
                     Uba1111I            CCGCGG                                                                       
                     Uba1113I            CCGCGG                                                                       
                     Uba1126I            CCGCGG                                                                       
                     Uba1187I            CCGCGG                                                                       
                     Uba1229I            CCGCGG                                                                       
                     Uba1234I            CCGCGG                                                                       
                     Uba1244I            CCGCGG                                                                       
                     Uba1306I            CCGCGG                                                                       
                     Uba1364I            CCGCGG                                                                       
                     Uba1369I            CCGCGG                                                                       
                     UbaHKAI             CCGCGG                                                                       
 SalI                                    G^TCGAC                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X                   
                     Acs1371I            GTCGAC                                                                       
                     Acs1372I            GTCGAC                                                                       
                     Acs1373I            GTCGAC                                                                       
                     Acs1421I            GTCGAC                                                                       
                     Acs1422I            GTCGAC                                                                       
                     BbrUII              GTCGAC                                                                       
                     BspMKI              G^TCGAC                                                                      
                     BstZ16I             GTCGAC                                                                       
                     BtgAI               GTCGAC                                                                       
                     CglAII              GTCGAC                                                                       
                     HgiCIII             G^TCGAC                                                                      
                     HgiDII              G^TCGAC                                                                      
                     KoyI                GTCGAC                                                                       
                     NopI                G^TCGAC                                                                      
                     Psp32I              GTCGAC                                                                       
                     Psp33I              GTCGAC                                                                       
                     Psp89I              GTCGAC                                                                       
                     RflFI               G^TCGAC                                                                      
                     RheI                GTCGAC                                                                       
                     RhpI                GTCGAC                                                                       
                     RrhI                GTCGAC                                                                       
                     Rrh4273I            G^TCGAC                                                                      
                     RroI                GTCGAC                                                                       
                     RtrI                G^TCGAC                                                                      
                     Rtr63I              G^TCGAC                                                                      
                     Uba89I              GTCGAC                                                                       
                     XamI                GTCGAC                                                                       
                     XciI                G^TCGAC                                                                      
 SanDI                                   GG^G(A/T)CCC           2                                                     
                     KflI                GG^G(A/T)CCC                 F                                               
                     Sse1825I            GG^G(A/T)CCC                                                                 
 SapI                                    GCTCTTC(1/4)           2     N                                               
                     BspQI               GCTCTTC(1/4)                 N                                               
                     LguI                GCTCTTC(1/4)                 F                                               
                     PciSI               GCTCTTC(1/4)                 I                                               
                     VpaK32I             GCTCTTC(1/4)                                                                 
 SauI                                    CC^TNAGG               2                                                     
                     AocI                CC^TNAGG                                                                     
                     AsuSAI              CCTNAGG                                                                      
                     AxyI                CC^TNAGG                     J                                               
                     Bli643I             CCTNAGG                                                                      
                     BliHKI              CC^TNAGG                                                                     
                     Bse21I              CC^TNAGG                     I,V                                             
                     BseG73I             CCTNAGG                                                                      
                     BspR7I              CC^TNAGG                                                                     
                     Bst29I              CCTNAGG                                                                      
                     Bst30I              CCTNAGG                                                                      
                     BstZ6I              CCTNAGG                                                                      
                     Bsu36I              CC^TNAGG                     N                                               
                     CvnI                CC^TNAGG                                                                     
                     EciCI               CCTNAGG                                                                      
                     Eco76I              CCTNAGG                                                                      
                     Eco81I              CC^TNAGG                     F,K                                             
                     Eco115I             CCTNAGG                                                                      
                     Eco118I             CCTNAGG                                                                      
                     Eco110kI            CCTNAGG                                                                      
                     Lmu60I              CC^TNAGG                                                                     
                     MstII               CC^TNAGG                                                                     
                     OxaNI               CC^TNAGG                                                                     
                     PxyMZI              CCTNAGG                                                                      
                     SauHI               CCTNAGG                                                                      
                     SecIII              CCTNAGG                                                                      
                     SshAI               CC^TNAGG                                                                     
                     Uba1184II           CCTNAGG                                                                      
                     Uba1294I            CCTNAGG                                                                      
                     Uba1332I            CCTNAGG                                                                      
                     Uba1333I            CCTNAGG                                                                      
 ScaI                                    AGT^ACT                2     B,C,F,J,K,M,N,O,Q,R,S,X                         
                     Acc113I             AGT^ACT                                                                      
                     AflIV               AGTACT                                                                       
                     Asp763I             AGTACT                                                                       
                     AssI                AGT^ACT                                                                      
                     BmcAI               AGT^ACT                      V                                               
                     Bpa34I              AGT^ACT                                                                      
                     BshHI               AGTACT                                                                       
                     BsoSI               AGTACT                                                                       
                     BstMI               AGTACT                                                                       
                     DpaI                AGT^ACT                                                                      
                     Eco255I             AGT^ACT                                                                      
                     LlaDI               AGTACT                                                                       
                     NmeSI               AGTACT                                                                       
                     PinI                AGTACT                                                                       
                     RflFII              AGT^ACT                                                                      
                     Uba1094I            AGTACT                                                                       
                     Uba1158I            AGTACT                                                                       
                     VchO49I             AGTACT                                                                       
                     ZrmI                AGT^ACT                      I                                               
 ScrFI                                   CC^NGG                 2     J,N                                             
                     Bme1390I            CC^NGG                       F                                               
                     BmrFI               CC^NGG                       V                                               
                     BsaCI               CCNGG                                                                        
                     BsoI                CCNGG                                                                        
                     Bsp53I              CCNGG                                                                        
                     Bsp73I              CCNGG                                                                        
                     Bsp548I             CCNGG                                                                        
                     BssKI               ^CCNGG                       N                                               
                     BstMZ611I           ^CCNGG                                                                       
                     BstSCI              ^CCNGG                       I                                               
                     DsaV                ^CCNGG                                                                       
                     Ecl18kI             ^CCNGG                                                                       
                     Eco43I              CCNGG                                                                        
                     Eco51II             CCNGG                                                                        
                     Eco80I              CCNGG                                                                        
                     Eco85I              CCNGG                                                                        
                     Eco93I              CCNGG                                                                        
                     Eco153I             CCNGG                                                                        
                     Eco200I             CCNGG                                                                        
                     Eco13kI             ^CCNGG                                                                       
                     Eco21kI             ^CCNGG                                                                       
                     Eco137kI            ^CCNGG                                                                       
                     HpyNI               CCNGG                                                                        
                     Kpn2kI              ^CCNGG                                                                       
                     LlaMI               CCNGG                                                                        
                     Msp67I              CC^NGG                                                                       
                     MspR9I              CC^NGG                       I                                               
                     SdeAII              ^CCNGG                                                                       
                     SsoII               ^CCNGG                                                                       
                     StyD4I              ^CCNGG                       N                                               
                     Uba17I              CCNGG                                                                        
                     Uba1391I            CCNGG                                                                        
 SdeAI                                   CAGRAG(21/19)          2                                                     
 SdeOSI                                  (11/13)GACNNNNRTGA(12/10)2                                                     
 SduI                                    G(A/G/T)GC(A/C/T)^C    2     F                                               
                     AocII               G(A/G/T)GC(A/C/T)^C                                                          
                     Bka1125I            G(A/G/T)GC(A/C/T)C                                                           
                     BmyI                G(A/G/T)GC(A/C/T)^C                                                          
                     BsoCI               G(A/G/T)GC(A/C/T)^C                                                          
                     Bsp1286I            G(A/G/T)GC(A/C/T)^C          J,K,N                                           
                     BspLS2I             G(A/G/T)GC(A/C/T)^C                                                          
                     BstZ15I             G(A/G/T)GC(A/C/T)C                                                           
                     HpyF36IV            G(A/G/T)GC(A/C/T)C                                                           
                     HpyF47I             G(A/G/T)GC(A/C/T)C                                                           
                     MhlI                G(A/G/T)GC(A/C/T)^C          I,V                                             
                     Mlu40I              G(A/G/T)GC(A/C/T)C                                                           
                     NspII               G(A/G/T)GC(A/C/T)^C                                                          
                     TseAI               G(A/G/T)GC(A/C/T)C                                                           
                     Uba1362I            G(A/G/T)GC(A/C/T)C                                                           
 SecI                                    C^CNNGG                2                                                     
                     BsaJI               C^CNNGG                      N                                               
                     BseDI               C^CNNGG                      F                                               
                     BsoKI               CCNNGG                                                                       
                     BssECI              C^CNNGG                      I                                               
                     BstZ10I             CCNNGG                                                                       
                     Hpy99IV             CCNNGG                                                                       
                     HpyF10III           CCNNGG                                                                       
                     HpyF61II            CCNNGG                                                                       
                     HpyF67IV            CCNNGG                                                                       
                     Uba1442I            CCNNGG                                                                       
 Sen1736II                               GATCAG                 2                                                     
 Sen13311III                             GATCAG                 2                                                     
 SenTFV                                  GATCAG                 2                                                     
 SetI                                    A(C/G)(C/G)T^          2     I                                               
 SexAI                                   A^CC(A/T)GGT           2     M,N                                             
                     CsiI                A^CC(A/T)GGT                 F                                               
                     MabI                A^CC(A/T)GGT                 I                                               
 SfaNI                                   GCATC(5/9)             2     I,N,V                                           
                     BhaI                GCATC                                                                        
                     BmsI                GCATC(5/9)                   F                                               
                     BscAI               GCATC(4/6)                                                                   
                     BscUI               GCATC                                                                        
                     BsmNI               GCATC                                                                        
                     BspST5I             GCATC(5/9)                                                                   
                     Bst19I              GCATC(4/6)                                                                   
                     CjeP338II           GCATC                                                                        
                     LweI                GCATC(5/9)                   F                                               
                     Mva1312II           GCATC                                                                        
                     PhaI                GCATC(5/9)                                                                   
 SfeI                                    C^TPuPyAG              2                                                     
                     Bco163I             CTPuPyAG                                                                     
                     BdiSI               C^TPuPyAG                                                                    
                     BfmI                C^TPuPyAG                    F                                               
                     BpcI                C^TPuPyAG                                                                    
                     BstSFI              C^TPuPyAG                    I                                               
                     BthP35I             C^TPuPyAG                                                                    
                     HpyF2I              CTPuPyAG                                                                     
                     LlaBI               C^TPuPyAG                                                                    
                     SfcI                C^TPuPyAG                    N                                               
                     Srl56DI             C^TPuPyAG                                                                    
 SfiI                                    GGCCNNNN^NGGCC         2     C,F,I,J,K,M,N,O,Q,R,S,V,X                       
                     SdiI                GGCCNNNN^NGGCC                                                               
 SgfI                                    GCGAT^CGC              2     R                                               
                     AsiSI               GCGAT^CGC                    I,N                                             
                     RgaI                GCGAT^CGC                    I                                               
                     SfaAI               GCGAT^CGC                    F                                               
 SgrAI                                   CPu^CCGGPyG            2     N                                               
                     PsuNI               CPuCCGGPyG                                                                   
                     SvoI                CPuCCGGPyG                                                                   
 SgrDI                                   CG^TCGACG              2     F                                               
 SimI                                    GGGTC(-3/0)            2                                                     
                     BssIMI              GGGTC(-3/0)                                                                  
                     BtsPI               GGGTC                                                                        
                     SaiI                GGGTC                                                                        
                     ShaI                GGGTC                                                                        
 SmaI                                    CCC^GGG                2     B,C,F,I,J,K,M,N,O,Q,R,S,U,V,X,Y                 
                     AhyI                C^CCGGG                                                                      
                     Cfr9I               C^CCGGG                      F                                               
                     CfrJ4I              CCC^GGG                                                                      
                     CphBI               CCCGGG                                                                       
                     EaeAI               C^CCGGG                                                                      
                     EclRI               C^CCGGG                                                                      
                     KteAI               CCCGGG                                                                       
                     Pac25I              C^CCGGG                                                                      
                     PaeBI               CCC^GGG                                                                      
                     PspAI               C^CCGGG                                                                      
                     PspALI              CCC^GGG                                                                      
                     TspMI               C^CCGGG                      N                                               
                     Uba1220I            CCCGGG                                                                       
                     Uba1393I            CCCGGG                                                                       
                     XcyI                C^CCGGG                                                                      
                     XmaI                C^CCGGG                      I,N,R,U,V                                       
                     XmaCI               C^CCGGG                                                                      
 SmlI                                    C^TPyPuAG              2     N                                               
                     Sau90I              CTPyPuAG                                                                     
                     Sau93I              CTPyPuAG                                                                     
                     Sau98I              CTPyPuAG                                                                     
                     Sau96mI             CTPyPuAG                                                                     
                     SmoI                C^TPyPuAG                    F                                               
 SnaI                                    GTATAC                 2                                                     
                     BspM90I             GTA^TAC                                                                      
                     BssNAI              GTA^TAC                      I,V                                             
                     Bst1107I            GTA^TAC                      F,K                                             
                     BstBSI              GTA^TAC                                                                      
                     BstZ17I             GTA^TAC                      N                                               
                     VchO25I             GTATAC                                                                       
                     XcaI                GTA^TAC                                                                      
 SnaBI                                   TAC^GTA                2     C,K,M,N,R                                       
                     Bpu1831I            TACGTA                                                                       
                     BstSNI              TAC^GTA                      I,V                                             
                     EciAI               TACGTA                                                                       
                     Eco105I             TAC^GTA                      F                                               
                     Eco158II            TACGTA                                                                       
                     SspJI               TACGTA                                                                       
                     SspM1I              TACGTA                                                                       
                     SspM2I              TACGTA                                                                       
                     Uba1240I            TACGTA                                                                       
 Sno506I                                 GGCCGAG                2                                                     
 SpeI                                    A^CTAGT                2     B,J,K,M,N,O,Q,R,S,U,X                           
                     AclNI               A^CTAGT                                                                      
                     AhlI                A^CTAGT                      I,V                                             
                     BcuI                A^CTAGT                      F                                               
 SphI                                    GCATG^C                2     B,C,I,J,K,M,N,O,Q,R,S,V,X                       
                     Asp5HI              GCATGC                                                                       
                     BbuI                GCATG^C                                                                      
                     BfuAII              GCATG^C                                                                      
                     BscOI               GCATGC                                                                       
                     Bsp121I             GCATGC                                                                       
                     BtgAII              GCATGC                                                                       
                     CglAI               GCATGC                                                                       
                     PaeI                GCATG^C                      F                                               
                     PaeCI               GCATGC                                                                       
                     PfaAIII             GCATG^C                                                                      
                     RspLKI              GCATG^C                                                                      
                     SpaHI               GCATG^C                                                                      
                     SpaXI               GCATGC                                                                       
                     Uba1162I            GCATGC                                                                       
                     Uba1226I            GCATGC                                                                       
                     VchO68I             GCATGC                                                                       
 SplI                                    C^GTACG                2                                                     
                     BpuB5I              C^GTACG                                                                      
                     BsiWI               C^GTACG                      N                                               
                     BsmWI               CGTACG                                                                       
                     BvuBI               C^GTACG                                                                      
                     MaeK81I             C^GTACG                                                                      
                     Pfl23II             C^GTACG                      F                                               
                     PfuNI               CGTACG                                                                       
                     PpuAI               C^GTACG                                                                      
                     PspLI               C^GTACG                      I                                               
                     SmaAI               CGTACG                                                                       
                     SplAI               CGTACG                                                                       
                     SspKI               CGTACG                                                                       
                     SunI                C^GTACG                                                                      
                     SpnD39IIIA          CACNNNNNNNCTT                                                                
                     SpnD39IIIB          CACNNNNNNNCTT                                                                
                     SpnD39IIIC          CACNNNNNNNCTT                                                                
                     SpnD39IIID          CACNNNNNNNCTT                                                                
 SpoDI                                   GCGGRAG                2                                                     
 SrfI                                    GCCC^GGGC              2                                                     
 Sse232I                                 CG^CCGGCG              2                                                     
                     MreI                CG^CCGGCG                    F                                               
 Sse8387I                                CCTGCA^GG              2     K                                               
                     SbfI                CCTGCA^GG                    I,N,V                                           
                     SdaI                CCTGCA^GG                    F                                               
 Sse8647I                                AG^G(A/T)CCT           2                                                     
 SspI                                    AAT^ATT                2     B,C,F,I,J,K,N,Q,R,U,V,X                         
 Ssp6803IV                               GAAGGC                 2                                                     
 SstE37I                                 CGAAGAC(20/18)         2                                                     
 Sth132I                                 CCCG(4/8)              2                                                     
                     NcuII               CCCG                                                                         
 StuI                                    AGG^CCT                2     B,J,K,M,N,Q,R,X                                 
                     AatI                AGG^CCT                                                                      
                     AehII               AGGCCT                                                                       
                     Asp78I              AGGCCT                                                                       
                     AspMI               AGG^CCT                                                                      
                     ChyI                AGGCCT                                                                       
                     Eco147I             AGG^CCT                      F                                               
                     Eco1524I            AGG^CCT                                                                      
                     GdiI                AGG^CCT                                                                      
                     GobAI               AGGCCT                                                                       
                     NtaSI               AGGCCT                                                                       
                     PceI                AGG^CCT                      I,V                                             
                     PluI                AGGCCT                                                                       
                     Pme55I              AGG^CCT                                                                      
                     Ppu13I              AGGCCT                                                                       
                     SarI                AGG^CCT                                                                      
                     Sru30DI             AGG^CCT                                                                      
                     SseBI               AGG^CCT                      C                                               
                     SsvI                AGGCCT                                                                       
                     SteI                AGG^CCT                                                                      
                     Uba40I              AGGCCT                                                                       
                     Uba1170I            AGGCCT                                                                       
                     Uba1180I            AGGCCT                                                                       
                     Uba1217I            AGGCCT                                                                       
                     Uba1239I            AGGCCT                                                                       
                     Uba1371I            AGGCCT                                                                       
                     Uba1403I            AGGCCT                                                                       
                     Uba1419I            AGGCCT                                                                       
                     VchO44I             AGGCCT                                                                       
 StyI                                    C^C(A/T)(A/T)GG        2     C,J,N                                           
                     BsmSI               C^C(A/T)(A/T)GG                                                              
                     BssT1I              C^C(A/T)(A/T)GG              I,V                                             
                     Bst224I             CC(A/T)(A/T)GG                                                               
                     CfrBI               C^C(A/T)(A/T)GG                                                              
                     Eco130I             C^C(A/T)(A/T)GG              F                                               
                     Eco208II            CC(A/T)(A/T)GG                                                               
                     EcoT14I             C^C(A/T)(A/T)GG              K                                               
                     EcoT104I            CC(A/T)(A/T)GG                                                               
                     ErhI                C^C(A/T)(A/T)GG              I                                               
                     ErhB9II             C^C(A/T)(A/T)GG                                                              
                     SblAI               CC(A/T)(A/T)GG                                                               
                     SblBI               CC(A/T)(A/T)GG                                                               
                     SblCI               CC(A/T)(A/T)GG                                                               
                     Uba1311I            CC(A/T)(A/T)GG                                                               
 Sty13348III                             GATCAG                 2                                                     
 StyLTI                                  CAGAG                  3                                                     
 StyLTIII                                GAGNNNNNNRTAYG         1                                                     
 StySBLI                                 CGANNNNNNTACC          1                                                     
                     StySENI             CGANNNNNNTACC                                                                
 StySEAI                                 ACANNNNNNTYCA          1                                                     
 StySGI                                  TAANNNNNNRTCG          1                                                     
 StySJI                                  GAGNNNNNNGTRC          1                                                     
 StySKI                                  CGATNNNNNNNGTTA        1                                                     
 StySPI                                  AACNNNNNNGTRC          1                                                     
 StySQI                                  AACNNNNNNRTAYG         1                                                     
 SwaI                                    ATTT^AAAT              2     J,M,N                                           
                     BstRZ246I           ATTT^AAAT                                                                    
                     BstSWI              ATTT^AAAT                                                                    
                     MspSWI              ATTT^AAAT                                                                    
                     SmiI                ATTT^AAAT                    F,I,K,V                                         
 TaqI                                    T^CGA                  2     B,C,F,I,J,K,M,N,Q,R,S,U,V,X                     
                     CviSIII             TCGA                                                                         
                     EsaBC3I             TC^GA                                                                        
                     HpyV                TCGA                                                                         
                     Hpy26II             TCGA                                                                         
                     HpyF14III           TCGA                                                                         
                     HpyF16I             TCGA                                                                         
                     HpyF23I             TCGA                                                                         
                     HpyF24I             TCGA                                                                         
                     HpyF26III           TCGA                                                                         
                     HpyF30I             TCGA                                                                         
                     HpyF35I             TCGA                                                                         
                     HpyF40II            TCGA                                                                         
                     HpyF42IV            TCGA                                                                         
                     HpyF45I             TCGA                                                                         
                     HpyF49I             TCGA                                                                         
                     HpyF52I             TCGA                                                                         
                     HpyF59III           TCGA                                                                         
                     HpyF62II            TCGA                                                                         
                     HpyF64I             TCGA                                                                         
                     HpyF65II            TCGA                                                                         
                     HpyF66IV            TCGA                                                                         
                     HpyF71I             TCGA                                                                         
                     HpyF73II            TCGA                                                                         
                     HpyJP26II           TCGA                                                                         
                     PpaAII              T^CGA                                                                        
                     Taq20I              TCGA                                                                         
                     Tbr51I              TCGA                                                                         
                     TfiA3I              TCGA                                                                         
                     TfiTok6A1I          TCGA                                                                         
                     TflI                TCGA                                                                         
                     Tsc4aI              TCGA                                                                         
                     Tsp32I              T^CGA                                                                        
                     Tsp32II             T^CGA                                                                        
                     Tsp358I             TCGA                                                                         
                     Tsp505I             TCGA                                                                         
                     Tsp510I             TCGA                                                                         
                     TspNI               TCGA                                                                         
                     Tth24I              TCGA                                                                         
                     TthHB8I             T^CGA                                                                        
                     TthRQI              TCGA                                                                         
 TaqII                                   GACCGA(11/9)           2     Q,X                                             
 TatI                                    (A/T)^GTAC(A/T)        2     F                                               
 TauI                                    GC(C/G)G^C             2     F                                               
                     AspCNI              GC(C/G)GC                                                                    
                     BspUI               GC(C/G)G^C                                                                   
                     CglI                GC(C/G)GC                                                                    
                     NgoFVII             GC(C/G)GC                                                                    
 TdeDII                                  ACCAGG                 2                                                     
 TfiI                                    G^A(A/T)TC             2     N                                               
                     PfeI                G^A(A/T)TC                   F                                               
 TseI                                    G^C(A/T)GC             2     N                                               
                     AceI                G^C(A/T)GC                                                                   
                     ApeKI               G^C(A/T)GC                   N                                               
                     Bce14579I           GC(A/T)GC                                                                    
                     SuiI                G^C(A/T)GC                                                                   
                     Taq52I              G^C(A/T)GC                                                                   
                     TneI                GC(A/T)GC                                                                    
                     TseBI               GC(A/T)GC                                                                    
 TsoI                                    TARCCA(11/9)           2                                                     
 Tsp45I                                  ^GT(C/G)AC             2     N                                               
                     Hpy8II              GT(C/G)AC                                                                    
                     Hpy51I              ^GT(C/G)AC                                                                   
                     Hpy99II             GT(C/G)AC                                                                    
                     HpyF1I              GT(C/G)AC                                                                    
                     HpyF4I              GT(C/G)AC                                                                    
                     HpyF6II             GT(C/G)AC                                                                    
                     HpyF9I              GT(C/G)AC                                                                    
                     HpyF13I             GT(C/G)AC                                                                    
                     HpyF25II            GT(C/G)AC                                                                    
                     HpyF31II            GT(C/G)AC                                                                    
                     HpyF34II            GT(C/G)AC                                                                    
                     HpyF35IV            GT(C/G)AC                                                                    
                     HpyF36I             GT(C/G)AC                                                                    
                     HpyF40III           GT(C/G)AC                                                                    
                     HpyF48I             GT(C/G)AC                                                                    
                     HpyF49II            GT(C/G)AC                                                                    
                     HpyF51I             GT(C/G)AC                                                                    
                     HpyF62III           GT(C/G)AC                                                                    
                     NmuCI               ^GT(C/G)AC                   F                                               
                     TseFI               ^GT(C/G)AC                   I                                               
 Tsp4CI                                  ACN^GT                 2                                                     
                     Bst4CI              ACN^GT                       I,V                                             
                     HpyCH4III           ACN^GT                       N                                               
                     HpyF5II             ACNGT                                                                        
                     HpyF9III            ACNGT                                                                        
                     HpyF12I             ACNGT                                                                        
                     HpyF20I             ACNGT                                                                        
                     HpyF22I             ACNGT                                                                        
                     HpyF35III           ACNGT                                                                        
                     HpyF40I             ACNGT                                                                        
                     HpyF41I             ACNGT                                                                        
                     HpyF42II            ACNGT                                                                        
                     HpyF46I             ACNGT                                                                        
                     HpyF48II            ACNGT                                                                        
                     HpyF51II            ACNGT                                                                        
                     HpyF54I             ACNGT                                                                        
                     HpyF55I             ACNGT                                                                        
                     HpyF56I             ACNGT                                                                        
                     HpyF58I             ACNGT                                                                        
                     HpyF62I             ACNGT                                                                        
                     HpyF64II            ACNGT                                                                        
                     HpyF65I             ACNGT                                                                        
                     HpyF68I             ACNGT                                                                        
                     HpyF69I             ACNGT                                                                        
                     HpyF74I             ACNGT                                                                        
                     TaaI                ACN^GT                       F                                               
 TspDTI                                  ATGAA(11/9)            2     Q,X                                             
 TspEI                                   ^AATT                  2                                                     
                     MluCI               ^AATT                        N                                               
                     Sse9I               ^AATT                        I,V                                             
                     TasI                ^AATT                        F                                               
                     TseCI               AATT                                                                         
                     Tsp509I             ^AATT                                                                        
 TspGWI                                  ACGGA(11/9)            2     Q,X                                             
 TspRI                                   CA(C/G)TGNN^           2     N                                               
                     TscAI               CA(C/G)TGNN^                 F                                               
 TssI                                    GAGNNNCTC              2                                                     
 TstI                                    (8/13)CACNNNNNNTCC(12/7)2                                                     
 TsuI                                    GCGAC                  2                                                     
 Tth111I                                 GACN^NNGTC             2     I,K,N,Q,V,X                                     
                     AspI                GACN^NNGTC                                                                   
                     AtsI                GACN^NNGTC                                                                   
                     BgiI                GACN^NNGTC                                                                   
                     FsuI                GACNNNGTC                                                                    
                     NtaI                GACNNNGTC                                                                    
                     PflFI               GACN^NNGTC                   N                                               
                     PsyI                GACN^NNGTC                   F                                               
                     SmaAII              GACNNNGTC                                                                    
                     SpaPI               GACNNNGTC                                                                    
                     SplII               GACNNNGTC                                                                    
                     SplAII              GACNNNGTC                                                                    
                     TelI                GACN^NNGTC                                                                   
                     TrsKTII             GACNNNGTC                                                                    
                     TspI                GACNNNGTC                                                                    
                     TteI                GACNNNGTC                                                                    
                     TtrI                GACNNNGTC                                                                    
 Tth111II                                CAARCA(11/9)           2                                                     
                     TthHB27I            CAARCA                                                                       
 UbaF9I                                  TACNNNNNRTGT           2                                                     
 UbaF11I                                 TCGTA                  2                                                     
 UbaF12I                                 CTACNNNGTC             2                                                     
 UbaF13I                                 GAGNNNNNNCTGG          2                                                     
 UbaF14I                                 CCANNNNNTCG            2                                                     
 UbaPI                                   CGAACG                 2                                                     
 VspI                                    AT^TAAT                2     F,I,R,V                                         
                     AseI                AT^TAAT                      J,N                                             
                     AsnI                AT^TAAT                                                                      
                     BpoAI               AT^TAAT                                                                      
                     CprJK722I           ATTAAT                                                                       
                     PbuMZI              ATTAAT                                                                       
                     PshBI               AT^TAAT                      K                                               
                     Srl8DI              ATTAAT                                                                       
                     Srl55DII            ATTAAT                                                                       
                     Srl65DI             ATTAAT                                                                       
                     Srr17I              ATTAAT                                                                       
                     Sru4DI              AT^TAAT                                                                      
 Vtu19109I                               CACRAYC                2                                                     
 WviI                                    CACRAG(21/19)          2                                                     
 XbaI                                    T^CTAGA                2     B,C,F,I,J,K,M,N,Q,R,S,U,V,X                     
                     BspAAII             T^CTAGA                                                                      
                     BspLU11II           TCTAGA                                                                       
                     BsrXI               TCTAGA                                                                       
                     Msp23I              TCTAGA                                                                       
                     UbaN19I             TCTAGA                                                                       
 XcmI                                    CCANNNNN^NNNNTGG       2     N                                               
 XhoI                                    C^TCGAG                2     B,F,J,K,M,N,O,Q,R,S,U,X                         
                     AbrI                C^TCGAG                                                                      
                     AehI                CTCGAG                                                                       
                     AerAI               CTCGAG                                                                       
                     AhyAI               CTCGAG                                                                       
                     AlwFII              CTCGAG                                                                       
                     Asp15I              CTCGAG                                                                       
                     Asp47I              CTCGAG                                                                       
                     Asp703I             CTCGAG                                                                       
                     BadI                CTCGAG                                                                       
                     BbfI                CTCGAG                                                                       
                     BbiIII              CTCGAG                                                                       
                     BluI                C^TCGAG                                                                      
                     Bsp92I              CTCGAG                                                                       
                     Bsp129I             CTCGAG                                                                       
                     Bsp139I             CTCGAG                                                                       
                     Bsp140I             CTCGAG                                                                       
                     Bsp141I             CTCGAG                                                                       
                     Bsp142I             CTCGAG                                                                       
                     BspAAI              C^TCGAG                                                                      
                     BssHI               C^TCGAG                                                                      
                     BstHI               CTCGAG                                                                       
                     BstLI               CTCGAG                                                                       
                     BstVI               C^TCGAG                                                                      
                     BsuMI               CTCGAG                                                                       
                     R1.BsuMI            CTCGAG                                                                       
                     R2.BsuMI            CTCGAG                                                                       
                     R3.BsuMI            CTCGAG                                                                       
                     BthI                CTCGAG                                                                       
                     CcrI                C^TCGAG                                                                      
                     CjaI                CTCGAG                                                                       
                     DdeII               CTCGAG                                                                       
                     DrdDI               CTCGAG                                                                       
                     Eae2I               CTCGAG                                                                       
                     EscI                C^TCGAG                                                                      
                     MavI                C^TCGAG                                                                      
                     McaI                CTCGAG                                                                       
                     MecI                CTCGAG                                                                       
                     MhaI                CTCGAG                                                                       
                     MlaAI               C^TCGAG                                                                      
                     MpuI                CTCGAG                                                                       
                     MrhI                CTCGAG                                                                       
                     MscAI               CTCGAG                                                                       
                     MsiI                CTCGAG                                                                       
                     Msp23II             CTCGAG                                                                       
                     OcoI                CTCGAG                                                                       
                     PaeR7I              C^TCGAG                      N                                               
                     PanI                C^TCGAG                                                                      
                     Pfl67I              CTCGAG                                                                       
                     PflNI               CTCGAG                                                                       
                     PflWI               CTCGAG                                                                       
                     Psp4I               CTCGAG                                                                       
                     PspNI               CTCGAG                                                                       
                     Sal1974I            CTCGAG                                                                       
                     Sau3239I            C^TCGAG                                                                      
                     SauLPII             C^TCGAG                                                                      
                     Sbi68I              C^TCGAG                                                                      
                     Sca1827I            CTCGAG                                                                       
                     SciI                CTC^GAG                                                                      
                     Sci1831I            CTCGAG                                                                       
                     SciBI               CTCGAG                                                                       
                     ScuI                CTCGAG                                                                       
                     SdiAI               CTCGAG                                                                       
                     SexI                CTCGAG                                                                       
                     Sfr274I             C^TCGAG                      I,V                                             
                     Sfu1762I            CTCGAG                                                                       
                     SgaI                CTCGAG                                                                       
                     SgoI                CTCGAG                                                                       
                     Sgr1841I            CTCGAG                                                                       
                     Shy1766I            CTCGAG                                                                       
                     SlaI                C^TCGAG                      C,Y                                             
                     SluI                CTCGAG                                                                       
                     Sol10179I           C^TCGAG                                                                      
                     SpaI                CTCGAG                                                                       
                     Sph1719I            CTCGAG                                                                       
                     SrifpI              CTCGAG                                                                       
                     Ssp4I               CTCGAG                                                                       
                     StaAI               CTCGAG                                                                       
                     StrI                C^TCGAG                                                                      
                     Sve194I             CTCGAG                                                                       
                     TliI                C^TCGAG                                                                      
                     Uba1130I            CTCGAG                                                                       
                     Uba1148I            CTCGAG                                                                       
                     Uba1154I            CTCGAG                                                                       
                     Uba1166I            CTCGAG                                                                       
                     Uba1237I            CTCGAG                                                                       
                     Uba1248I            CTCGAG                                                                       
                     Uba1271I            CTCGAG                                                                       
                     Uba1298I            CTCGAG                                                                       
                     Uba1335I            CTCGAG                                                                       
                     Uba1397I            CTCGAG                                                                       
                     Uba1448I            CTCGAG                                                                       
                     XpaI                C^TCGAG                                                                      
 XhoII                                   Pu^GATCPy              2                                                     
                     AitII               PuGATCPy                                                                     
                     AitAI               PuGATCPy                                                                     
                     Asp17I              PuGATCPy                                                                     
                     Asp22I              PuGATCPy                                                                     
                     Asp1HI              PuGATCPy                                                                     
                     Asp6HI              PuGATCPy                                                                     
                     Asp8HI              PuGATCPy                                                                     
                     Asp14HI             PuGATCPy                                                                     
                     Asp21HI             PuGATCPy                                                                     
                     BloHI               Pu^GATCPy                                                                    
                     BpuGI               PuGATCPy                                                                     
                     BscSI               PuGATCPy                                                                     
                     BstX2I              Pu^GATCPy                    I,V                                             
                     BstYI               Pu^GATCPy                    N                                               
                     DsaIII              Pu^GATCPy                                                                    
                     MflI                Pu^GATCPy                    K                                               
                     MspNII              Pu^GATCPy                                                                    
                     PsuI                Pu^GATCPy                    F                                               
                     Tru201I             Pu^GATCPy                                                                    
                     Uba1432I            PuGATCPy                                                                     
 XmaIII                                  C^GGCCG                2                                                     
                     AaaI                C^GGCCG                                                                      
                     BseX3I              C^GGCCG                      I,V                                             
                     BsoDI               CGGCCG                                                                       
                     BstZI               C^GGCCG                      R                                               
                     EagI                C^GGCCG                      N                                               
                     EclXI               C^GGCCG                      S                                               
                     Eco52I              C^GGCCG                      F,K                                             
                     SenPT16I            C^GGCCG                                                                      
                     TauII               CGGCCG                                                                       
                     Tsp504I             CGGCCG                                                                       
 XmnI                                    GAANN^NNTTC            2     N,R                                             
                     Asp700I             GAANN^NNTTC                  M                                               
                     BbvAI               GAANN^NNTTC                                                                  
                     MroXI               GAANN^NNTTC                  I,V                                             
                     PdmI                GAANN^NNTTC                  F                                               
                     Psc2I               GAANNNNTTC                                                                   
                     SynII               GAANNNNTTC                                                                   



